RRC ID 38817
著者 Takenouchi T, Munekata E.
タイトル beta-Amyloid peptide, substance P, and SEC receptor ligand activate cytoplasmic Ca2+ in neutrophil-like HL-60 cells: effect of chemotactic peptide antagonist BocMLF.
ジャーナル Peptides
Abstract It has been reported that a discrete peptide fragment of beta-amyloid protein, beta A(25-35), and neuropeptide substance P (SP) possessed sequence homology and could bind to the serine protease inhibitor (serpin) enzyme complex (SEC) receptor. Thus, it has been thought that these peptides and SEC receptor ligand might have similar biological activities. In the present study, we found that C-terminal amidated beta A(25-35)-NH2, SP, and the SEC receptor ligand, Phe-Val-Phe-Leu-Met(FVFLM), could induce an increase in the intracellular free Ca2+ concentration ([Ca2+]i) in neutrophil-like human leukemic (HL-60) cells. Pretreatment with pertussis toxin (PTX) potently inhibited the increase in [Ca2+]i stimulated by these peptides, suggesting that these responses might be mediated by PTX-sensitive G-proteins. Furthermore, we examined the effect on these responses of t-butyloxycarbonyl-methionyl-leucyl-phenylalanine (BocMLF), which is a competitive antagonist of chemotactic peptide N-formyl-methionyl-leucyl-phenylalanine (fMLF) at its receptor. BocMLF scarcely inhibited the [Ca2+]i increase stimulated by beta A(25-35)-NH2. However, the increase in FVFLM-induced [Ca2+]i was potently inhibited by BocMLF. The results suggest that the [Ca2+]i activation of beta A(25-35)-NH2 may have a different mechanism from that of FVFLM in neutrophil-like HL-60 cells, which is not mediated by the SEC-receptor.
巻・号 16(6)
ページ 1019-24
公開日 1995-1-1
DOI 10.1016/0196-9781(95)00084-w
PII 0196-9781(95)00084-W
PMID 8532582
MeSH Amino Acid Sequence Amyloid beta-Peptides / chemistry Amyloid beta-Peptides / pharmacology Calcium / metabolism* Cell Line Cytoplasm / metabolism Humans Ligands Molecular Sequence Data N-Formylmethionine Leucyl-Phenylalanine / antagonists & inhibitors* Neutrophils / drug effects* Neutrophils / metabolism* Oligopeptides / chemistry Oligopeptides / pharmacology* Peptide Fragments / chemistry Peptide Fragments / pharmacology Receptors, Cell Surface / metabolism Substance P / pharmacology
IF 2.843
引用数 16
WOS 分野 PHARMACOLOGY & PHARMACY ENDOCRINOLOGY & METABOLISM BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 HL60(RCB0041)