| RRC ID |
38837
|
| 著者 |
Okada N, Fushimi M, Nagata Y, Fukunaga T, Tsutsumi Y, Nakagawa S, Mayumi T.
|
| タイトル |
Evaluation of angiogenic inhibitors with an in vivo quantitative angiogenesis method using agarose microencapsulation and mouse hemoglobin enzyme-linked immunosorbent assay.
|
| ジャーナル |
Jpn J Cancer Res
|
| Abstract |
In the present work, using a previously reported in vivo quantitative tumor-angiogenesis model, we attempted to ascertain whether this animal model is suitable for practical use in monitoring inhibitors of tumor angiogenesis. Mouse sarcoma-180 cells, human A431 cells or rat C6 cells microencapsulated in agarose beads were implanted s.c. into C57BL/6 mice. The level of blood vessel induction at the agarose pellet site was evaluated using mouse hemoglobin enzyme-linked immunosorbent assay on day 10 after implantation. Hydrocortisone, tetrahydro-S, medroxyprogesterone acetate, pentosan polysulfate and suramin inhibited blood vessel growth in our in vivo tumor-angiogenesis assay system, and heparin enhanced the antiangiogenic effects of hydrocortisone and tetrahydro-S. These results are almost entirely consistent with those observed in common assay systems, and suggest that this method may be useful for the identification and quantitative evaluation of inhibitors of tumor angiogenesis.
|
| 巻・号 |
87(9)
|
| ページ |
952-7
|
| 公開日 |
1996-9-1
|
| DOI |
10.1111/j.1349-7006.1996.tb02125.x
|
| PII |
0910505096859023
|
| PMID |
8878458
|
| PMC |
PMC5921206
|
| MeSH |
Animals
Carcinoma, Squamous Cell / blood supply*
Carcinoma, Squamous Cell / drug therapy*
Cortodoxone / analogs & derivatives
Cortodoxone / pharmacology
Drug Compounding
Drug Evaluation, Preclinical
Enzyme-Linked Immunosorbent Assay
Female
Hemoglobins*
Humans
Hydrocortisone / pharmacology
Male
Medroxyprogesterone / pharmacology
Mice
Mice, Inbred C57BL
Neovascularization, Pathologic / drug therapy*
Pentosan Sulfuric Polyester / pharmacology
Sarcoma 180 / blood supply*
Sarcoma 180 / drug therapy*
Sepharose*
Suramin / pharmacology
Tumor Cells, Cultured
|
| 引用数 |
10
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
A431(RCB0202)
HL60(RCB0041) |
