| RRC ID |
38838
|
| 著者 |
Shindoh M, Higashino F, Kaya M, Yasuda M, Funaoka K, Hanzawa M, Hida K, Kohgo T, Amemiya A, Yoshida K, Fujinaga K.
|
| タイトル |
Correlated expression of matrix metalloproteinases and ets family transcription factor E1A-F in invasive oral squamous-cell-carcinoma-derived cell lines.
|
| ジャーナル |
Am J Pathol
|
| Abstract |
Matrix metalloproteinases (MMPs) have been implicated in invasion and metastasis of tumor cells. Transcription regulatory regions of MMP genes often contain binding sites for ets transcription factors. We recently isolated a cDNA encoding human E1A-F, a member of the ets oncogene family, and showed that E1A-F can upregulate MMP genes by CAT assay. We attempted to investigate the relationship between E1A-F mRNA expression and MMP protein expression in four different types of oral squamous-cell-carcinoma-derived cell lines (HSC 3, SAS, KB, and Ca 9.22). HSC 3 and SAS are highly invasive cell lines when they are injected in the tongue of nude mice. Raft culture of HSC 3 and SAS revealed the same characteristics as seen in tumors implanted in vivo. Both type I collagenase (MMP-1) and 92-kd type IV collagenase (MMP-9) were detected in cultured HSC 3 and SAS cells. E1A-F mRNA was demonstrated to be highly expressed in HSC 3 and SAS by Northern blotting, and in situ hybridization confirmed E1A-F mRNA expression at the invasion front of tumor cells seeded on collagen gel. On the other hand, KB and Ca 9.22 have little potential for invasion, and MMP-1 and MMP-9 protein and E1A-F mRNA could not be detected. These results suggest that the ets-related E1A-F participates in the regulation of invasion-associated MMP genes and is involved in presenting invasive activity in tumor cells of oral squamous cell carcinoma.
|
| 巻・号 |
148(3)
|
| ページ |
693-700
|
| 公開日 |
1996-3-1
|
| PMID |
8774124
|
| PMC |
PMC1861731
|
| MeSH |
Blotting, Northern
Carcinoma, Squamous Cell / metabolism*
Carcinoma, Squamous Cell / pathology
Extracellular Matrix / metabolism*
Humans
Immunohistochemistry
In Situ Hybridization
Metalloendopeptidases / metabolism*
Mouth Neoplasms / metabolism*
Mouth Neoplasms / pathology
Neoplasm Invasiveness
Proto-Oncogene Proteins / metabolism*
Proto-Oncogene Proteins c-ets
Transcription Factors / metabolism*
Tumor Cells, Cultured
|
| IF |
3.491
|
| 引用数 |
58
|
|
WOS 分野
|
PATHOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
MRC-5 |
