| RRC ID |
38848
|
| 著者 |
Nagamune H, Ohnishi C, Katsuura A, Fushitani K, Whiley RA, Tsuji A, Matsuda Y.
|
| タイトル |
Intermedilysin, a novel cytotoxin specific for human cells secreted by Streptococcus intermedius UNS46 isolated from a human liver abscess.
|
| ジャーナル |
Infect Immun
|
| Abstract |
A novel cytotoxin (intermedilysin) specific for human cells was identified as a cytolytic factor of Streptococcus intermedius UNS46 isolated from a human liver abscess. Intermedilysin caused human cell death with membrane blebs. Intermedilysin was purified from UNS46 culture medium by means of gel filtration and hydrophobic chromatography. The purified toxin was resolved into major and minor bands of 54 and 53 kDa, respectively, by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. These proteins reacted with an antibody against intermedilysin. Five internal peptide fragments of intermedilysin were sequenced and found to have 42 to 71% homology with the thiol-activated cytotoxin pneumolysin. However, the action of intermedilysin differed from that of thiol-activated cytotoxins, especially in terms of a lack of activation by dithiothreitol and resistance to treatments with N-ethylmaleimide and 5,5'-dithio-bis-(2-nitrobenzoic acid), although cholesterol inhibited the toxin activity. Intermedilysin was potently hemolytic on human erythrocytes but was 100-fold less effective on chimpanzee and cynomolgus monkey erythrocytes. Intermedilysin was not hemolytic in nine other animal species tested. Since human erythrocytes treated with trypsin were far less sensitive to intermedilysin than were the intact cells, a cell membrane protein(s) may participate in the intermedilysin action. These data demonstrated that intermedilysin is distinguishable from all known bacterial cytolysins.
|
| 巻・号 |
64(8)
|
| ページ |
3093-100
|
| 公開日 |
1996-8-1
|
| DOI |
10.1128/iai.64.8.3093-3100.1996
|
| PMID |
8757839
|
| PMC |
PMC174193
|
| MeSH |
Amino Acid Sequence
Animals
Bacterial Proteins*
Bacterial Toxins / isolation & purification
Bacterial Toxins / toxicity*
Bacteriocins
Cats
Cattle
Cell Membrane / drug effects
Cells, Cultured
Chickens
Cytokines / isolation & purification
Cytokines / metabolism
Cytokines / toxicity*
Cytotoxins / isolation & purification
Cytotoxins / metabolism
Cytotoxins / toxicity*
Dogs
Dose-Response Relationship, Drug
Humans
Hydrogen-Ion Concentration
Liver Abscess / microbiology
Macaca fascicularis
Mice
Molecular Sequence Data
Pan troglodytes
Rats
Salts / pharmacology
Sequence Analysis
Sequence Homology, Amino Acid
Species Specificity
Streptococcus / chemistry*
Streptococcus / isolation & purification
Streptococcus / metabolism
Streptococcus / pathogenicity
Sulfhydryl Reagents
Temperature
Trypsin
|
| IF |
3.201
|
| 引用数 |
108
|
|
WOS 分野
|
INFECTIOUS DISEASES
IMMUNOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
NB69(RCB0480)
JM(RCB0537) |
