論文 - 詳細
| RRC ID | 38871 |
|---|---|
| 著者 | Nagashima H. |
| タイトル | Rubratoxin-B-induced secretion of chemokine ligands of cysteine-cysteine motif chemokine receptor 5 (CCR5) and its dependence on heat shock protein 90 in HL60 cells. |
| ジャーナル | Environ Toxicol Pharmacol |
| Abstract |
To elucidate the mechanism underlying rubratoxin B toxicity, the effects of rubratoxin B on the secretion of CCR5 chemokines, CCL3, CCL4, and CCL5, in a human promyelocytic leukemia cell line, HL60, were investigated. In addition, to examine whether the molecular chaperone 90-kDa heat shock protein (Hsp90) contributes to rubratoxin B toxicity, the effects of Hsp90-specific inhibitors, radicicol and geldanamycin, were investigated. Exposure to rubratoxin B for 24h induced secretion of each CCR5 chemokine, although the effect on CCL5 secretion was modest, and it enhanced secretion of proinflammatory cytokines tumor necrosis factor-α, CXCL8, and CCL2. Concomitant treatment with radicicol abolished the rubratoxin-induced secretion of all cytokines investigated. Geldanamycin antagonized the rubratoxin B-induced effects on CCL3 and CCL5, but not CCL4; the effects of geldanamycin were less than that of radicicol. Taken together, the results suggest that rubratoxin B, with the contribution of Hsp90, induces secretion of CCR5 chemokines. |
| 巻・号 | 40(3) |
| ページ | 997-1000 |
| 公開日 | 2015-11-1 |
| DOI | 10.1016/j.etap.2015.10.012 |
| PII | S1382-6689(15)30114-9 |
| PMID | 26595743 |
| MeSH | Benzoquinones / pharmacology Chemokine CCL3 / metabolism Chemokine CCL4 / metabolism Chemokine CCL5 / metabolism Chemokines / metabolism* Gene Expression Regulation / drug effects HL-60 Cells HSP90 Heat-Shock Proteins / antagonists & inhibitors HSP90 Heat-Shock Proteins / metabolism* Humans Lactams, Macrocyclic / pharmacology Macrolides / pharmacology Mycotoxins / toxicity* Receptors, CCR5 / metabolism* |
| IF | 3.292 |
| 引用数 | 2 |
| WOS 分野 | PHARMACOLOGY & PHARMACY ENVIRONMENTAL SCIENCES TOXICOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | HL60(RCB0041) |