RRC ID 38881
著者 Higuchi H, Shoji T, Murase Y, Iijima S, Nishijima K.
タイトル Siglec-9 modulated IL-4 responses in the macrophage cell line RAW264.
ジャーナル Biosci Biotechnol Biochem
Abstract Siglecs, an immunoglobulin-like lectin family that recognizes the sialic acid moiety, regulate various aspects of immune responses. In the present study, we investigated the effects of Siglecs on the macrophage cell line RAW264, which was stimulated with interleukin-4 (IL-4). The induction of arginase-1 (Arg1) by IL-4 was stronger in Siglec-9-expressing cells than in mock cells. Mutations in the cytoplasmic tyrosine-based inhibitory motifs in Siglec-9 markedly reduced the expression of Arg1. The phosphorylation of Akt by IL-4 and extracellular signal-regulated kinase (ERK) without IL-4 was stronger in Siglec-9-expressing cells, indicating the enhanced activation of the phosphatidylinositol 3 kinase (PI-3K) and mitogen-activated protein kinase kinase (MEK)/ERK pathways, respectively. The enhanced expression of Arg1 was inhibited by MEK inhibitors, but not by PI-3K inhibitor. These results indicate that Siglec-9 affects several different signaling pathways in IL-4-stimulated macrophages, which resulted in enhanced induction of Arg1 in Siglec-9-expressing RAW264 cells.
巻・号 80(3)
ページ 501-9
公開日 2016-1-1
DOI 10.1080/09168451.2015.1104238
PMID 26540411
MeSH Animals Antigens, CD Antigens, Differentiation, B-Lymphocyte Arginase / metabolism Cell Line Cells, Cultured Humans Interleukin-4 / physiology* MAP Kinase Signaling System Macrophages / immunology* Mice Phosphatidylinositol 3-Kinases / metabolism Phosphorylation STAT6 Transcription Factor / metabolism
IF 1.516
引用数 7
WOS 分野 FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY CHEMISTRY, APPLIED
リソース情報
ヒト・動物細胞 RAW 264(RCB0535)