RRC ID 38883
Author Goto T, Naknukool S, Yoshitake R, Hanafusa Y, Tokiwa S, Li Y, Sakamoto T, Nitta T, Kim M, Takahashi N, Yu R, Daiyasu H, Seno S, Matsuda H, Kawada T.
Title Proinflammatory cytokine interleukin-1β suppresses cold-induced thermogenesis in adipocytes.
Journal Cytokine
Abstract In this study, we investigated the effects of interleukin-1β (IL-1β), a typical proinflammatory cytokine on the β-adrenoreceptor-stimulated induction of uncoupling protein 1 (UCP1) expression in adipocytes. IL-1β mRNA expression levels were upregulated in white adipose tissues of obese mice and in RAW264.7 macrophages under conditions designed to mimic obese adipose tissue. Isoproterenol-stimulated induction of UCP1 mRNA expression was significantly inhibited in C3H10T1/2 adipocytes by conditioned medium from lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages in comparison with control conditioned medium. This inhibition was significantly attenuated in the presence of recombinant IL-1 receptor antagonist and IL-1β antibody, suggesting that activated macrophage-derived IL-1β is an important cytokine for inhibition of β-adrenoreceptor-stimulated UCP1 induction in adipocytes. IL-1β suppressed isoproterenol-induced UCP1 mRNA expression in C3H10T1/2 adipocytes, and this effect was partially but significantly abrogated by inhibition of extracellular signal-regulated kinase (ERK). IL-1β also suppressed the isoproterenol-induced activation of the UCP1 promoter and transcription factors binding to the cAMP response element. Moreover, intraperitoneal administration of IL-1β suppressed cold-induced UCP1 expression in adipose tissues. These findings suggest that IL-1β upregulated in obese adipose tissues suppresses β-adrenoreceptor-stimulated induction of UCP1 expression through ERK activation in adipocytes.
Volume 77
Pages 107-14
Published 2016-1
DOI 10.1016/j.cyto.2015.11.001
PII S1043-4666(15)30089-2
PMID 26556104
MeSH 3T3-L1 Cells Adipocytes / drug effects Adipocytes / metabolism* Adipose Tissue, White / metabolism Adrenergic beta-Agonists / pharmacology Animals Cell Line Cold Temperature* Culture Media, Conditioned / pharmacology Enzyme Activation / drug effects Extracellular Signal-Regulated MAP Kinases / metabolism Gene Expression / drug effects Immunoblotting Inflammation Mediators / metabolism* Inflammation Mediators / pharmacology Interleukin-1beta / genetics Interleukin-1beta / metabolism* Interleukin-1beta / pharmacology Ion Channels / genetics Ion Channels / metabolism Isoproterenol / pharmacology Lipopolysaccharides / pharmacology Macrophages / drug effects Macrophages / metabolism Male Mice Mice, Inbred C57BL Mitochondrial Proteins / genetics Mitochondrial Proteins / metabolism Obesity / genetics Obesity / metabolism Reverse Transcriptase Polymerase Chain Reaction Uncoupling Protein 1
IF 3.514
Times Cited 8
WOS Category IMMUNOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
Resource
Human and Animal Cells