論文 - 詳細
| RRC ID | 38883 |
|---|---|
| 著者 | Goto T, Naknukool S, Yoshitake R, Hanafusa Y, Tokiwa S, Li Y, Sakamoto T, Nitta T, Kim M, Takahashi N, Yu R, Daiyasu H, Seno S, Matsuda H, Kawada T. |
| タイトル | Proinflammatory cytokine interleukin-1β suppresses cold-induced thermogenesis in adipocytes. |
| ジャーナル | Cytokine |
| Abstract |
In this study, we investigated the effects of interleukin-1β (IL-1β), a typical proinflammatory cytokine on the β-adrenoreceptor-stimulated induction of uncoupling protein 1 (UCP1) expression in adipocytes. IL-1β mRNA expression levels were upregulated in white adipose tissues of obese mice and in RAW264.7 macrophages under conditions designed to mimic obese adipose tissue. Isoproterenol-stimulated induction of UCP1 mRNA expression was significantly inhibited in C3H10T1/2 adipocytes by conditioned medium from lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages in comparison with control conditioned medium. This inhibition was significantly attenuated in the presence of recombinant IL-1 receptor antagonist and IL-1β antibody, suggesting that activated macrophage-derived IL-1β is an important cytokine for inhibition of β-adrenoreceptor-stimulated UCP1 induction in adipocytes. IL-1β suppressed isoproterenol-induced UCP1 mRNA expression in C3H10T1/2 adipocytes, and this effect was partially but significantly abrogated by inhibition of extracellular signal-regulated kinase (ERK). IL-1β also suppressed the isoproterenol-induced activation of the UCP1 promoter and transcription factors binding to the cAMP response element. Moreover, intraperitoneal administration of IL-1β suppressed cold-induced UCP1 expression in adipose tissues. These findings suggest that IL-1β upregulated in obese adipose tissues suppresses β-adrenoreceptor-stimulated induction of UCP1 expression through ERK activation in adipocytes. |
| 巻・号 | 77 |
| ページ | 107-14 |
| 公開日 | 2016-1-1 |
| DOI | 10.1016/j.cyto.2015.11.001 |
| PII | S1043-4666(15)30089-2 |
| PMID | 26556104 |
| MeSH | 3T3-L1 Cells Adipocytes / drug effects Adipocytes / metabolism* Adipose Tissue, White / metabolism Adrenergic beta-Agonists / pharmacology Animals Cell Line Cold Temperature* Culture Media, Conditioned / pharmacology Enzyme Activation / drug effects Extracellular Signal-Regulated MAP Kinases / metabolism Gene Expression / drug effects Immunoblotting Inflammation Mediators / metabolism* Inflammation Mediators / pharmacology Interleukin-1beta / genetics Interleukin-1beta / metabolism* Interleukin-1beta / pharmacology Ion Channels / genetics Ion Channels / metabolism Isoproterenol / pharmacology Lipopolysaccharides / pharmacology Macrophages / drug effects Macrophages / metabolism Male Mice Mice, Inbred C57BL Mitochondrial Proteins / genetics Mitochondrial Proteins / metabolism Obesity / genetics Obesity / metabolism Reverse Transcriptase Polymerase Chain Reaction Uncoupling Protein 1 |
| IF | 2.952 |
| 引用数 | 33 |
| WOS 分野 | IMMUNOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |