Reference - Detail
| RRC ID | 38998 |
|---|---|
| Author | Kawaguchi Y, Tanaka H, Okada T, Konishi H, Takahashi M, Ito M, Asai J. |
| Title | Effect of reactive oxygen species on the elastin mRNA expression in cultured human dermal fibroblasts. |
| Journal | Free Radic Biol Med |
| Abstract |
The effects of ultraviolet A (UVA) and reactive oxygen species (ROS), generated by a xanthine and xanthine oxidase (XOD) system, on the mRNA expression of elastin, were investigated using cultured human dermal fibroblasts. Total RNA was isolated and subjected to Northern blot analysis using synthesized 530 base cDNA probe for elastin with primers derived from exon 10 and 1 of human elastin. UVA irradiation did not affect elastin mRNA expression. In contrast, ROS resulted in a dose-related increase in the level of elastin mRNA up to 1.8-fold in cultured human dermal fibroblasts. Catalase, used as scavenger, essentially prevented the ROS induced alterations in elastin mRNA levels. These results suggest that ROS produced in the dermis may contribute to elastin deposition observed in photoaging skin. |
| Volume | 23(1) |
| Pages | 162-5 |
| Published | 1997-1-1 |
| DOI | 10.1016/s0891-5849(96)00570-9 |
| PII | S0891-5849(96)00570-9 |
| PMID | 9165309 |
| MeSH | Blotting, Northern Catalase / pharmacology Cells, Cultured Elastin / genetics* Fibroblasts Gene Expression Regulation Humans RNA, Messenger / genetics RNA, Messenger / metabolism* Reactive Oxygen Species / metabolism* Skin / metabolism* Skin / radiation effects Skin Aging / genetics Skin Aging / physiology* Ultraviolet Rays Xanthine Xanthine Oxidase / metabolism Xanthines / metabolism |
| IF | 6.17 |
| Times Cited | 49 |
| WOS Category | ENDOCRINOLOGY & METABOLISM BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 5 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | NB1RGB(RCB0222) |