| RRC ID |
38998
|
| Author |
Kawaguchi Y, Tanaka H, Okada T, Konishi H, Takahashi M, Ito M, Asai J.
|
| Title |
Effect of reactive oxygen species on the elastin mRNA expression in cultured human dermal fibroblasts.
|
| Journal |
Free Radic Biol Med
|
| Abstract |
The effects of ultraviolet A (UVA) and reactive oxygen species (ROS), generated by a xanthine and xanthine oxidase (XOD) system, on the mRNA expression of elastin, were investigated using cultured human dermal fibroblasts. Total RNA was isolated and subjected to Northern blot analysis using synthesized 530 base cDNA probe for elastin with primers derived from exon 10 and 1 of human elastin. UVA irradiation did not affect elastin mRNA expression. In contrast, ROS resulted in a dose-related increase in the level of elastin mRNA up to 1.8-fold in cultured human dermal fibroblasts. Catalase, used as scavenger, essentially prevented the ROS induced alterations in elastin mRNA levels. These results suggest that ROS produced in the dermis may contribute to elastin deposition observed in photoaging skin.
|
| Volume |
23(1)
|
| Pages |
162-5
|
| Published |
1997-1-1
|
| DOI |
10.1016/s0891-5849(96)00570-9
|
| PII |
S0891-5849(96)00570-9
|
| PMID |
9165309
|
| MeSH |
Blotting, Northern
Catalase / pharmacology
Cells, Cultured
Elastin / genetics*
Fibroblasts
Gene Expression Regulation
Humans
RNA, Messenger / genetics
RNA, Messenger / metabolism*
Reactive Oxygen Species / metabolism*
Skin / metabolism*
Skin / radiation effects
Skin Aging / genetics
Skin Aging / physiology*
Ultraviolet Rays
Xanthine
Xanthine Oxidase / metabolism
Xanthines / metabolism
|
| IF |
6.17
|
| Times Cited |
49
|
|
WOS Category
|
ENDOCRINOLOGY & METABOLISM
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
NB1RGB(RCB0222) |
