RRC ID 39012
著者 Okahashi N, Nakamura I, Jimi E, Koide M, Suda T, Nishihara T.
タイトル Specific inhibitors of vacuolar H(+)-ATPase trigger apoptotic cell death of osteoclasts.
ジャーナル J Bone Miner Res
Abstract Osteoclasts are multinucleated bone-resorbing cells that play a critical role in bone remodeling. Specific inhibitors of vacuolar H(+)-ATPase (V-ATPase), concanamycin A and bafilomycin A1, abolish bone resorption by osteoclasts. In this study, we examined whether these V-ATPase inhibitors trigger apoptotic cell death in osteoclasts, using murine osteoclast-like multinucleated cells (OCLs) formed in vitro. Acridine orange staining revealed that the treatment of OCLs with concanamycin A resulted in chromatin condensation and alterations in nuclear morphology within a few hours. The TdT-mediated dUTP-nick-end labeling (TUNEL) reaction confirmed the apoptotic features of OCLs treated with concanamycin A. The accelerated apoptotic cell death induced by concanamycin A occurred in OCLs treated with interleukin-1 alpha or macrophage colony-stimulating factor as well, which are known to elongate the survival time of osteoclasts. In contrast, these inhibitors did not induce cell death of osteoblastic cells isolated from mouse calvaria. These results suggest that functional impairment of V-ATPase triggers apoptotic cell death in osteoclasts.
巻・号 12(7)
ページ 1116-23
公開日 1997-7-1
DOI 10.1359/jbmr.1997.12.7.1116
PMID 9200012
MeSH Animals Anti-Bacterial Agents / pharmacology Apoptosis / drug effects* Cells, Cultured Enzyme Inhibitors / pharmacology* Interleukin-1 / pharmacology Kinetics Macrolides* Macrophage Colony-Stimulating Factor / pharmacology Mice Osteoblasts / cytology Osteoblasts / drug effects Osteoblasts / enzymology Osteoclasts / cytology Osteoclasts / drug effects* Osteoclasts / enzymology* Proton-Translocating ATPases / antagonists & inhibitors* Vacuolar Proton-Translocating ATPases*
IF 5.854
引用数 61
WOS 分野 ENDOCRINOLOGY & METABOLISM
リソース情報
ヒト・動物細胞 MC3T3-E1(RCB1126)