RRC ID 39038
Author Iwata E, Ikeda S, Matsunaga S, Kurata M, Yoshioka Y, Criqui MC, Genschik P, Ito M.
Title GIGAS CELL1, a novel negative regulator of the anaphase-promoting complex/cyclosome, is required for proper mitotic progression and cell fate determination in Arabidopsis.
Journal Plant Cell
Abstract Increased cellular ploidy is widespread during developmental processes of multicellular organisms, especially in plants. Elevated ploidy levels are typically achieved either by endoreplication or endomitosis, which are often regarded as modified cell cycles that lack an M phase either entirely or partially. We identified GIGAS CELL1 (GIG1)/OMISSION OF SECOND DIVISION1 (OSD1) and established that mutation of this gene triggered ectopic endomitosis. On the other hand, it has been reported that a paralog of GIG1/OSD1, UV-INSENSITIVE4 (UVI4), negatively regulates endoreplication onset in Arabidopsis thaliana. We showed that GIG1/OSD1 and UVI4 encode novel plant-specific inhibitors of the anaphase-promoting complex/cyclosome (APC/C) ubiquitin ligase. These proteins physically interact with APC/C activators, CDC20/FZY and CDH1/FZR, in yeast two-hybrid assays. Overexpression of CDC20.1 and CCS52B/FZR3 differentially promoted ectopic endomitosis in gig1/osd1 and premature occurrence of endoreplication in uvi4. Our data suggest that GIG1/OSD1 and UVI4 may prevent an unscheduled increase in cellular ploidy by preferentially inhibiting APC/C(CDC20) and APC/C(FZR), respectively. Generation of cells with a mixed identity in gig1/osd1 further suggested that the APC/C may have an unexpected role for cell fate determination in addition to its role for proper mitotic progression.
Volume 23(12)
Pages 4382-93
Published 2011-12-1
DOI 10.1105/tpc.111.092049
PII tpc.111.092049
PMID 22167058
PMC PMC3269872
MeSH Alleles Anaphase-Promoting Complex-Cyclosome Arabidopsis / cytology Arabidopsis / genetics* Arabidopsis / growth & development Arabidopsis / metabolism Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Cdc20 Proteins Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Cell Nucleus / genetics Cell Nucleus / metabolism Chromosomes, Plant / genetics Chromosomes, Plant / metabolism Cloning, Molecular Cotyledon / genetics Cotyledon / metabolism Cyclin B / genetics Cyclin B / metabolism Cytokinesis Enzyme Inhibitors / metabolism Gene Expression Regulation, Plant Genes, Plant Genetic Complementation Test Mitosis* Mutation Plant Cells / metabolism Plant Epidermis / cytology Plant Epidermis / genetics Plant Epidermis / growth & development Plant Epidermis / metabolism Plant Stomata / cytology Plant Stomata / genetics Plant Stomata / growth & development Plant Stomata / metabolism Plants, Genetically Modified / cytology Plants, Genetically Modified / genetics Plants, Genetically Modified / growth & development Plants, Genetically Modified / metabolism Plasmids / genetics Plasmids / metabolism Ploidies Pollen / growth & development Pollen / metabolism Protein Interaction Mapping Transcription Factors / genetics Transcription Factors / metabolism Transcriptional Activation Two-Hybrid System Techniques Ubiquitin-Protein Ligase Complexes / genetics Ubiquitin-Protein Ligase Complexes / metabolism*
IF 9.618
Times Cited 66
Arabidopsis / Cultured plant cells, genes pst15307