論文 - 詳細
| RRC ID | 39056 |
|---|---|
| 著者 | Monzen S, Chiba M, Hosokawa Y. |
| タイトル | Genetic network profiles associated with established resistance to ionizing radiation in acute promyelocytic leukemia cells and their extracellular vesicles. |
| ジャーナル | Oncol Rep |
| Abstract |
Radiation-resistant acute promyelocytic leukemia (APL) cells present challenges to treatment, and the acquisition of resistance to ionizing radiation (IR) is a matter of clinical concern. However, little information is available on the behavior of radio-resistant APL in terms of gene expression profiles and intercellular communication. In this study, cDNA microarray and RT-PCR were used to analyze the intracellular genetic network and extracellular vesicles (EVs), respectively, in the established radio-resistant HL60 (Res-HL60) cell line. Significant changes in the expression of 7,309 known mRNAs were observed in Res-HL60 relative to control. In addition, 7 mRNAs were determined as targets because significant changes in the expression were observed using Ingenuity analysis software, confirming the quantitative RT-PCR. However, EVs from Res-HL60 cells did not include these target molecules. These results suggest that radio-resistant APL is regulated by the expression and suppression of specific molecules, and these molecules are not transferred between cells by EVs. |
| 巻・号 | 35(2) |
| ページ | 749-56 |
| 公開日 | 2016-2-1 |
| DOI | 10.3892/or.2015.4471 |
| PMID | 26718911 |
| MeSH | Extracellular Vesicles / genetics* Gene Regulatory Networks / genetics* HL-60 Cells Humans Leukemia, Promyelocytic, Acute / genetics* Oligonucleotide Array Sequence Analysis Radiation Tolerance / genetics* Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Transcriptome |
| IF | 3.417 |
| 引用数 | 0 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HL60(RCB0041) |