論文 - 詳細
| RRC ID | 3907 |
|---|---|
| 著者 | Kobayashi F, Maeta E, Terashima A, Kawaura K, Ogihara Y, Takumi S. |
| タイトル | Development of abiotic stress tolerance via bZIP-type transcription factor LIP19 in common wheat. |
| ジャーナル | J Exp Bot |
| Abstract |
Cereal lip19 genes encoding bZIP-type transcription factors are assumed to play a regulatory role in gene expression during the cold acclimation process. However, no direct evidence shows an association of LIP19-type bZIPs with stress tolerance or activation of stress-responsive Cor/Lea genes. To understand the molecular basis of development of abiotic stress tolerance through the LIP19 transcription factor, a wheat lip19 homologue, Wlip19, was isolated and characterized. Wlip19 expression was activated by low temperature in seedlings and was higher in a freezing-tolerant cultivar than in a freezing-sensitive one. Wlip19 also responded to drought and exogenous ABA treatment. Wlip19-expressing transgenic tobacco showed a significant increase in abiotic stress tolerance, especially freezing tolerance. Expression of a GUS reporter gene under the control of promoter sequences of four wheat Cor/Lea genes, Wdhn13, Wrab17, Wrab18, and Wrab19, was enhanced by Wlip19 expression in wheat callus and tobacco plants. These results indicate that WLIP19 acts as a transcriptional regulator of Cor/Lea genes in the development of abiotic stress tolerance. Moreover, direct protein-protein interaction between WLIP19 and a wheat OBF1 homologue TaOBF1, another bZIP-type transcription factor, was observed, suggesting that this interaction is conserved in cereals. |
| 巻・号 | 59(4) |
| ページ | 891-905 |
| 公開日 | 2008-1-1 |
| DOI | 10.1093/jxb/ern014 |
| PII | ern014 |
| PMID | 18326864 |
| MeSH | Abscisic Acid / pharmacology Acclimatization Basic-Leucine Zipper Transcription Factors / genetics Basic-Leucine Zipper Transcription Factors / metabolism Cold Temperature Freezing Gene Expression Profiling Gene Expression Regulation, Plant / physiology Germination / drug effects Osmolar Concentration Phylogeny Plant Proteins / genetics Plant Proteins / metabolism* Tobacco / metabolism Transcription Factors / genetics Transcription Factors / metabolism* Triticum / genetics Triticum / metabolism* |
| IF | 5.908 |
| 引用数 | 60 |
| WOS 分野 | PLANT SCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 情報 | KOMUGI(EST database) |
| コムギ | LPGKU2269(Chinese Spring) |