RRC ID 3907
著者 Kobayashi F, Maeta E, Terashima A, Kawaura K, Ogihara Y, Takumi S.
タイトル Development of abiotic stress tolerance via bZIP-type transcription factor LIP19 in common wheat.
ジャーナル J Exp Bot
Abstract Cereal lip19 genes encoding bZIP-type transcription factors are assumed to play a regulatory role in gene expression during the cold acclimation process. However, no direct evidence shows an association of LIP19-type bZIPs with stress tolerance or activation of stress-responsive Cor/Lea genes. To understand the molecular basis of development of abiotic stress tolerance through the LIP19 transcription factor, a wheat lip19 homologue, Wlip19, was isolated and characterized. Wlip19 expression was activated by low temperature in seedlings and was higher in a freezing-tolerant cultivar than in a freezing-sensitive one. Wlip19 also responded to drought and exogenous ABA treatment. Wlip19-expressing transgenic tobacco showed a significant increase in abiotic stress tolerance, especially freezing tolerance. Expression of a GUS reporter gene under the control of promoter sequences of four wheat Cor/Lea genes, Wdhn13, Wrab17, Wrab18, and Wrab19, was enhanced by Wlip19 expression in wheat callus and tobacco plants. These results indicate that WLIP19 acts as a transcriptional regulator of Cor/Lea genes in the development of abiotic stress tolerance. Moreover, direct protein-protein interaction between WLIP19 and a wheat OBF1 homologue TaOBF1, another bZIP-type transcription factor, was observed, suggesting that this interaction is conserved in cereals.
巻・号 59(4)
ページ 891-905
公開日 2008-1-1
DOI 10.1093/jxb/ern014
PII ern014
PMID 18326864
MeSH Abscisic Acid / pharmacology Acclimatization Basic-Leucine Zipper Transcription Factors / genetics Basic-Leucine Zipper Transcription Factors / metabolism Cold Temperature Freezing Gene Expression Profiling Gene Expression Regulation, Plant / physiology Germination / drug effects Osmolar Concentration Phylogeny Plant Proteins / genetics Plant Proteins / metabolism* Tobacco / metabolism Transcription Factors / genetics Transcription Factors / metabolism* Triticum / genetics Triticum / metabolism*
IF 5.908
引用数 60
WOS 分野 PLANT SCIENCES
リソース情報
情報 KOMUGI(EST database)
コムギ LPGKU2269(Chinese Spring)