| RRC ID |
39107
|
| 著者 |
Kanda T, Foucand L, Nakamura Y, Niot I, Besnard P, Fujita M, Sakai Y, Hatakeyama K, Ono T, Fujii H.
|
| タイトル |
Regulation of expression of human intestinal bile acid-binding protein in Caco-2 cells.
|
| ジャーナル |
Biochem J
|
| Abstract |
Molecular mechanisms of the bile acid active transport system in the ileal enterocytes remain unknown. We examined whether bile acids affect human enterocyte gene expression of intestinal bile acid-binding protein (I-BABP), a component of this transport system. Differentiated Caco-2 cells were incubated in the presence of human bile, bile acids or other lipids. The level of I-BABP expression was evaluated by Northern and Western blot analyses. A 24 h incubation of Caco-2 cells in a medium containing either bile or bile acids resulted in a remarkable 7.5-fold increase in the I-BABP mRNA level over the control level. Neither cholesterol, palmitic acid, phosphatidylcholine nor cholestyramine treated bile showed any difference in I-BABP mRNA expression from the control. Bile acid treatment increased the level of I-BABP mRNA in Caco-2 cells in a time- and dose-dependent manner. Western blot analysis showed that this induction led to increase in cytosolic I-BABP. Chenodeoxycholic acid and deoxycholic acid showed greater induction effects than other hydrophilic bile acids, including their own glycine conjugates. Pretreatment by actinomycin D or cycloheximide completely inhibited the up-regulation of I-BABP expression by bile acid. Bile acids, especially lipophilic bile acids, increase the I-BABP expression in Caco-2-cells, suggesting that luminal bile acids play an important role in regulating the I-BABP gene expression.
|
| 巻・号 |
330 ( Pt 1)(Pt 1)
|
| ページ |
261-5
|
| 公開日 |
1998-2-15
|
| DOI |
10.1042/bj3300261
|
| PMID |
9461519
|
| PMC |
PMC1219136
|
| MeSH |
Bile Acids and Salts / physiology*
Caco-2 Cells / metabolism*
Carrier Proteins / genetics
Carrier Proteins / metabolism*
Cytosol / metabolism
Dose-Response Relationship, Drug
Fatty Acid-Binding Protein 7
Fatty Acid-Binding Proteins
Female
Gene Expression Regulation, Neoplastic
Humans
Hydroxysteroid Dehydrogenases*
Ileum / physiology*
Membrane Glycoproteins*
Middle Aged
Myelin P2 Protein / genetics
Neoplasm Proteins*
Protein Synthesis Inhibitors / pharmacology
RNA, Messenger / genetics
RNA, Neoplasm / genetics
Transcription, Genetic / drug effects
Tumor Suppressor Proteins*
Up-Regulation
|
| IF |
4.097
|
| 引用数 |
71
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
CACO-2(RCB0988) |
