RRC ID 39116
Author Shimoyama Y, Shibata T, Kitajima M, Hirohashi S.
Title Molecular cloning and characterization of a novel human classic cadherin homologous with mouse muscle cadherin.
Journal J Biol Chem
Abstract We used a novel cDNA cloning method based on the cadherin-beta-catenin protein interaction and identified a new human classic-type cadherin, which we named cadherin-15, from adult brain and skeletal muscle cDNA libraries. Sequence analysis revealed that this cadherin was closely related to mouse muscle cadherin and seemed to be its human counterpart. However, its deduced amino acid sequence differed from that of mouse muscle cadherin in that it had an extra 31-amino acid sequence at its C terminus that has been found neither in mouse muscle cadherin nor in any other known classic cadherin. Analysis of cadherin-15 protein expressed in L fibroblasts showed that it was cleaved proteolytically, expressed on the cell surfaces as a mature form of about 124-kDa, and functioned as a cell-cell adhesion molecule in a homophilic and specific manner, but Ca2+ did not protect it against degradation by trypsin. Our findings also suggest that cadherin-15 mediates cell-cell adhesion with a binding strength comparable to that of E-cadherin.
Volume 273(16)
Pages 10011-8
Published 1998-4-17
DOI 10.1074/jbc.273.16.10011
PII S0021-9258(18)46230-8
PMID 9545347
MeSH Adult Amino Acid Sequence Animals Base Sequence Brain / metabolism* Cadherins / biosynthesis* Cadherins / chemistry* Cadherins / genetics Cell Adhesion Cell Adhesion Molecules / chemistry Cell Adhesion Molecules / metabolism Cell Aggregation Cloning, Molecular Humans L Cells Mice Molecular Sequence Data Molecular Weight Muscle, Skeletal / metabolism* Peptide Fragments / chemistry Peptide Fragments / isolation & purification Recombinant Proteins / biosynthesis Recombinant Proteins / chemistry Sequence Homology Transfection
IF 4.238
Times Cited 16
Human and Animal Cells LTK-(RCB0208)