RRC ID 39118
Author Ikeda M, Tsuji N, Kikukawa K, Asahara Y, Nakashima A, Minatogawa Y.
Title DNA-binding properties of the overexpressed recombinant estrogen receptor alpha.
Journal Biochem. Mol. Biol. Int.
Abstract Estrogen receptor alpha was overexpressed in COS-7 cells by transformation of an expression vector with the full length open reading frame of the receptor alpha. The nuclear estradiol-receptor complex and the soluble receptor from the COS-7 cells were cross-linked with an estrogen response element (ERE), which was substituted with 5-bromo-2'-deoxyuridine (BrdUVRE), as the receptor dimers by UV irradiation. In gel retardation analysis, the specific bindings of both nuclear and soluble receptors to ERE were decreased with increasing of KCl concentration compared with 0.1 M KCl. The ionic interactions of both receptors with ERE are thought to be similar.
Volume 45(4)
Pages 673-80
Published 1998-7
DOI 10.1080/15216549800203072
PMID 9713689
MeSH Animals Antibodies, Monoclonal Bromodeoxyuridine COS Cells DNA / genetics DNA / metabolism* Dimerization Estradiol / metabolism Estrogen Receptor alpha Gene Expression Potassium Chloride / pharmacology Receptors, Estrogen / chemistry Receptors, Estrogen / genetics Receptors, Estrogen / immunology Receptors, Estrogen / metabolism* Recombinant Proteins / metabolism Regulatory Sequences, Nucleic Acid* Solubility Ultraviolet Rays
Times Cited 2
Human and Animal Cells