論文 - 詳細
| RRC ID | 39131 |
|---|---|
| 著者 | Yasuda H, Shima N, Nakagawa N, Mochizuki SI, Yano K, Fujise N, Sato Y, Goto M, Yamaguchi K, Kuriyama M, Kanno T, Murakami A, Tsuda E, Morinaga T, Higashio K. |
| タイトル | Identity of osteoclastogenesis inhibitory factor (OCIF) and osteoprotegerin (OPG): a mechanism by which OPG/OCIF inhibits osteoclastogenesis in vitro. |
| ジャーナル | Endocrinology |
| Abstract |
The morphogenesis and remodeling of bone depends on the integrated activity of osteoblasts that form bone and osteoclasts that resorb bone. We previously reported the isolation of a new cytokine termed osteoclastogenesis inhibitory factor, OCIF, which specifically inhibits osteoclast development. Here we report the cloning of a complementary DNA of human OCIF. OCIF is identical to osteoprotegerin (OPG), a soluble member of the tumor-necrosis factor receptor family that inhibits osteoclastogenesis. Recombinant human OPG/OCIF specifically acts on bone tissues and increases bone mineral density and bone volume associated with a decrease of active osteoclast number in normal rats. Osteoblasts or bone marrow-derived stromal cells support osteoclastogenesis through cell-to-cell interactions. A single class of high affinity binding sites for OPG/OCIF appears on a mouse stromal cell line, ST2, in response to 1,25-dihydroxyvitamin D3. An anti-OPG/OCIF antibody that blocks the binding abolishes the biological activity of OPG/OCIF. When the sites are blocked with OPG/OCIF, ST2 cells fail to support osteoclastogenesis. These results suggest that the sites are involved in cell-to-cell signaling between stromal cells and osteoclast progenitors and that OPG/OCIF inhibits osteoclastogenesis by interrupting the signaling through the sites. |
| 巻・号 | 139(3) |
| ページ | 1329-37 |
| 公開日 | 1998-3-1 |
| DOI | 10.1210/endo.139.3.5837 |
| PMID | 9492069 |
| MeSH | Amino Acid Sequence Animals Bone Density / drug effects Bone Remodeling Cells, Cultured Cloning, Molecular Glycoproteins / genetics Glycoproteins / physiology* Humans Male Mice Molecular Sequence Data Osteoclasts / drug effects Osteoclasts / physiology* Osteoprotegerin Rats Rats, Sprague-Dawley Receptors, Cytoplasmic and Nuclear* Receptors, Tumor Necrosis Factor / genetics Receptors, Tumor Necrosis Factor / physiology* Recombinant Proteins / pharmacology |
| IF | 3.934 |
| 引用数 | 944 |
| WOS 分野 | ENDOCRINOLOGY & METABOLISM |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 60 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) ST2(RCB0224) |