| RRC ID |
39132
|
| Author |
Kanai F, Kawakami T, Hamada H, Sadata A, Yoshida Y, Tanaka T, Ohashi M, Tateishi K, Shiratori Y, Omata M.
|
| Title |
Adenovirus-mediated transduction of Escherichia coli uracil phosphoribosyltransferase gene sensitizes cancer cells to low concentrations of 5-fluorouracil.
|
| Journal |
Cancer Res
|
| Abstract |
5-fluorouracil (5-FU), although a widely used chemotherapeutic agent, has a limited effect in the treatment of human solid tumors due to their resistance to the cytotoxic effects of 5-FU. Escherichia coli uracil phosphoribosyltransferase (UPRT) is a pyrimidine salvage enzyme that catalyzes the synthesis of UMP from uracil and 5-phosphoribosyl-alpha-1-diphosphate. The present study demonstrates that adenovirus-mediated transduction of E. coli UPRT gene results in marked sensitization of colon, gastric, liver, and pancreas cancer cell lines to low concentration of 5-FU in vitro. The in vitro bystander effect was observed when only 10% of the hepatoma Hep3B cells were infected with UPRT-expressing adenovirus. In addition, 5-FU treatment of human hepatoma or gastric cancer xenografts in nude mice transduced with UPRT was demonstrated to result in significant in vivo antitumor effects. The adenovirus vector transduction of the UPRT gene followed by 5-FU administration is representative of a new chemosensitization strategy for cancer gene therapy.
|
| Volume |
58(9)
|
| Pages |
1946-51
|
| Published |
1998-5-1
|
| PMID |
9581837
|
| MeSH |
Adenoviridae / genetics*
Animals
Blotting, Northern
Cells, Cultured
DNA Primers / chemistry
Escherichia coli / enzymology*
Fluorouracil / pharmacology*
Gene Expression Regulation, Enzymologic
Genes, Bacterial*
Genetic Therapy
Humans
Mice
Mice, Inbred BALB C
Mice, Nude
Neoplasms, Experimental / drug therapy
Neoplasms, Experimental / enzymology*
Pentosyltransferases / genetics*
Transfection*
Tumor Cells, Cultured / drug effects
Tumor Cells, Cultured / enzymology*
|
| IF |
9.727
|
| Times Cited |
91
|
|
WOS Category
|
ONCOLOGY
|
| Resource |
| Human and Animal Cells |
|
