RRC ID 39282
Author Kimura M, Matsuda Y, Yoshioka T, Okano Y.
Title Cell cycle-dependent expression and centrosome localization of a third human aurora/Ipl1-related protein kinase, AIK3.
Journal J Biol Chem
Abstract We earlier isolated cDNAs encoding novel human protein kinases AIK and AIK2 sharing high amino acid sequence identities with Drosophila Aurora and Saccharomyces cerevisiae Ipl1 kinases whose mutations cause abnormal chromosome segregation. In the present study, a third human cDNA (AIK3) highly homologous to aurora/IPL1 was isolated, and the nucleotide sequence was determined. This cDNA encodes 309 amino acids with a predicted molecular mass of 35.9 kDa. C-terminal kinase domain of AIK3 protein shares high amino acid sequence identities with those of Aurora/Ipl1 family protein kinases including human AIK, human AIK2, Xenopus pEg2, Drosophila Aurora, and yeast Ipl1, whereas the N-terminal domain of AIK3 protein shares little homology with any other Aurora/Ipl1 family members. AIK3 gene was assigned to human chromosome 19q13.43, which is a frequently deleted or rearranged region in several tumor tissues, by fluorescence in situ hybridization, somatic cell hybrid panel, and radiation hybrid cell panel. Northern blot analyses revealed that AIK3 expression was limited to testis. The expression levels of AIK3 in several cancer cell lines were elevated severalfold compared with normal fibroblasts. In HeLa cells, the endogenous AIK3 protein level is low in G1/S, accumulates during G2/M, and reduces after mitosis. Immunofluorescence studies using a specific antibody have shown that AIK3 is localized to centrosome during mitosis from anaphase to cytokinesis. These results suggest that AIK3 may play a role(s) in centrosome function at later stages of mitosis.
Volume 274(11)
Pages 7334-40
Published 1999-3-12
DOI 10.1074/jbc.274.11.7334
PMID 10066797
MeSH Amino Acid Sequence Aurora Kinase B Aurora Kinases Base Sequence Blotting, Western Cell Cycle* Centrosome / enzymology* Chromosome Mapping Chromosomes, Human, Pair 19 Cloning, Molecular DNA, Complementary HeLa Cells Humans Male Molecular Sequence Data Protein-Serine-Threonine Kinases / genetics* Protein-Serine-Threonine Kinases / metabolism Sequence Homology, Amino Acid Subcellular Fractions / enzymology Testis / enzymology Tumor Cells, Cultured
IF 4.238
Times Cited 211
Human and Animal Cells