論文 - 詳細
| RRC ID | 39286 |
|---|---|
| 著者 | Nomura R, Fujimoto T. |
| タイトル | Tyrosine-phosphorylated caveolin-1: immunolocalization and molecular characterization. |
| ジャーナル | Mol Biol Cell |
| Abstract |
Caveolin-1 was discovered as a major substrate for v-Src, but the effect of its tyrosine phosphorylation has not been known. We generated a specific antibody (PY14) to caveolin-1 phosphorylated at tyrosine 14 and studied the significance of the modification. By Western blotting of lysates of v-Src-expressing cells, PY14 recognized not only a 22-kDa band (the position of nonphosphorylated caveolin-1) but bands at 23-24 and 25 kDa. Bands of slower mobility were diminished by dephosphorylation and were also observed for mutant caveolin-1 lacking tyrosine 14. By immunofluorescence microscopy, PY14 did not label normal cells but detected large dots in v-Src-expressing cells. Immunoelectron microscopy revealed that the dots corresponded to aggregated caveolae and/or vesicles of various sizes; besides, the label was observed in intramembrane particle-free areas in the plasma membrane, which appeared to have been formed by fusion of flattened caveolae. A positive reaction with PY14 was found in normal cells after vanadate or pervanadate treatment; it occurred mainly at 22 kDa by Western blotting and was not seen as large dots by immunofluorescence microscopy. Detergent solubility, oligomerization, and association with caveolin-2 were observed similarly for caveolin-1 in normal and v-Src-expressing cells. The results indicate that phosphorylation of caveolin-1 in v-Src-expressing cells occurs at multiple residues and induces flattening, aggregation, and fusion of caveolae and/or caveolae-derived vesicles. |
| 巻・号 | 10(4) |
| ページ | 975-86 |
| 公開日 | 1999-4-1 |
| DOI | 10.1091/mbc.10.4.975 |
| PMID | 10198051 |
| PMC | PMC25222 |
| MeSH | Animals Caveolin 1 Caveolin 2 Caveolins* Cell Line Cell Line, Transformed Freeze Fracturing Humans Immunohistochemistry Kidney Membrane Proteins / analysis Membrane Proteins / biosynthesis Membrane Proteins / metabolism* Microscopy, Immunoelectron Phosphorylation Phosphotyrosine / metabolism Protein Binding Rats Recombinant Proteins / analysis Recombinant Proteins / biosynthesis Recombinant Proteins / metabolism Transfection |
| IF | 3.791 |
| 引用数 | 102 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 3Y1-B clone 1-6(RCB0488) NRK(RCB0043) SR-3Y1 |