論文 - 詳細
RRC ID | 39288 |
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著者 | Kitani T, Ishida A, Okuno S, Takeuchi M, Kameshita I, Fujisawa H. |
タイトル | Molecular cloning of Ca2+/calmodulin-dependent protein kinase phosphatase. |
ジャーナル | J Biochem |
Abstract |
Calmodulin-dependent protein kinase (CaM-kinase) phosphatase dephosphorylates and concomitantly deactivates CaM-kinase II activated upon autophosphorylation, and CaM-kinases IV and I activated upon phosphorylation by CaM-kinase kinase [Ishida, I., Okuno, S., Kitani, T., Kameshita, I., and Fujisawa, H. (1998) Biochem. Biophys. Res. Commun. 253, 159-163], suggesting that CaM-kinase phosphatase plays important roles in the function of Ca2+ in the cell, because the three multifunctional CaM-kinases (CaM-kinases I, II, and IV) are thought to be the key enzymes in the Ca2+-signaling system. In the present study, cDNA for CaM-kinase phosphatase was cloned from a rat brain cDNA library. The coded protein consisted of 450 amino acids with a molecular weight of 49, 165. Western blot analysis showed the ubiquitous tissue distribution of CaM-kinase phosphatase. Immunocytochemical analysis revealed that CaM-kinase phosphatase is evenly distributed outside the nucleus in a cell. |
巻・号 | 125(6) |
ページ | 1022-8 |
公開日 | 1999-6-1 |
DOI | 10.1093/oxfordjournals.jbchem.a022381 |
PMID | 10348902 |
MeSH | Amino Acid Sequence Animals Base Sequence Brain / enzymology Cloning, Molecular DNA, Complementary / genetics Escherichia coli / genetics Humans Mitogen-Activated Protein Kinases / metabolism Molecular Sequence Data Molecular Weight Protein Tyrosine Phosphatases / chemistry Protein Tyrosine Phosphatases / genetics* Protein Tyrosine Phosphatases / metabolism Rabbits Rats Recombinant Proteins / chemistry Recombinant Proteins / genetics Recombinant Proteins / metabolism Sequence Homology, Amino Acid Species Specificity Subcellular Fractions / enzymology Tissue Distribution |
IF | 2.476 |
引用数 | 50 |
WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
リソース情報 | |
ヒト・動物細胞 |