RRC ID 3928
著者 Wada S, Hamada M, Kobayashi K, Satoh N.
タイトル Novel genes involved in canonical Wnt/beta-catenin signaling pathway in early Ciona intestinalis embryos.
ジャーナル Dev Growth Differ
Abstract We report here characterization of five genes for novel components of the canonical Wnt/beta-catenin signaling pathway. These genes were identified in the ascidian Ciona intestinalis through a loss-of-function screening for genes required for embryogenesis with morpholinos, and four of them have counterparts in vertebrates. The five genes we studied are as follows: Ci-PGAP1, a Ciona orthologue of human PGAP1, which encodes GPI (glycosylphosphatidylinositol) inositol-deacylase, Ci-ZF278, a gene encoding a C2H2 zinc-finger protein, Ci-C10orf11, a Ciona orthologue of human C10orf11 that encodes a protein with leucine-rich repeats, Ci-Spatial/C4orf17, a single counterpart for two human genes Spatial and C4orf17, and Ci-FLJ10634, a Ciona orthologue of human FLJ10634 that encodes a member of the J-protein family. Knockdown of each of the genes mimicked beta-catenin knockdown and resulted in suppression of the expression of beta-catenin downstream genes (Ci-FoxD, Ci-Lhx3, Ci-Otx and Ci-Fgf9/16/20) and subsequent endoderm formation. For every gene, defects in knockdown embryos were rescued by overexpression of a constitutively active form, but not wild-type, of Ci-beta-catenin. Dosage-sensitive interactions were found between Ci-beta-catenin and each of the genes. These results suggest that these five genes act upstream of or parallel to Ci-beta-catenin in the Wnt/beta-catenin signaling pathway in early Ciona embryos.
巻・号 50(4)
ページ 215-27
公開日 2008-5-1
DOI 10.1111/j.1440-169X.2008.01012.x
PII DGD1012
PMID 18336583
MeSH Amino Acid Sequence Animals Ciona intestinalis / embryology* Ciona intestinalis / genetics Ciona intestinalis / metabolism Down-Regulation Embryo, Nonmammalian / metabolism* Gene Expression Regulation, Developmental Molecular Sequence Data Phosphoric Monoester Hydrolases Signal Transduction Wnt Proteins / genetics Wnt Proteins / metabolism* beta Catenin / genetics beta Catenin / metabolism*
IF 1.723
引用数 15
WOS 分野 DEVELOPMENTAL BIOLOGY CELL BIOLOGY
リソース情報
カタユウレイボヤ・(ニッポンウミシダ) Wild C.int