| RRC ID |
39295
|
| 著者 |
Shimoyama Y, Takeda H, Yoshihara S, Kitajima M, Hirohashi S.
|
| タイトル |
Biochemical characterization and functional analysis of two type II classic cadherins, cadherin-6 and -14, and comparison with E-cadherin.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
Classic cadherins can be grouped based on their deduced primary structures. Among them the type I cadherins have been well characterized; however, little is known about non-type I cadherins. In this study we characterized two human type II cadherins, cadherin-6 and cadherin-14, using a cDNA transfection system. They were each detected as two bands electrophoretically, were expressed on the external cell surface at cell-cell contact sites, and were associated with caten- ins. Direct sequencing of the N-terminal amino acids showed that the two bands of cadherin-14 corresponded to precursor and mature forms, whereas the two bands of cadherin-6 both had the N-terminal sequence of the mature form. Unlike type I cadherins, both cadherin-6 and -14 were not protected from trypsin degradation by Ca2+. We evaluated their adhesive functions by a long term cell aggregation method. The results suggest that both cadherin-6 and -14 have cell-cell binding strengths virtually equivalent to that of E-cadherin and that their binding specificities are distinct from that of E-cadherin. Cadherin-6 and -14 interacted with each other in an incomplete manner. They have a QAI tripeptide in the first extracellular subdomain instead of the HAV motif that is characteristic of type I cadherins and is intimately involved in the adhesive function. The QAI tripeptide, however, appeared not to be involved in the adhesive functions of cadherin-6 and -14.
|
| 巻・号 |
274(17)
|
| ページ |
11987-94
|
| 公開日 |
1999-4-23
|
| DOI |
10.1074/jbc.274.17.11987
|
| PII |
S0021-9258(19)73521-2
|
| PMID |
10207020
|
| MeSH |
Amino Acid Sequence
Animals
Cadherins / metabolism*
Cell Aggregation
Cells, Cultured
DNA, Complementary
Humans
Immunohistochemistry
Mice
|
| IF |
4.238
|
| 引用数 |
29
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
