| RRC ID |
3930
|
| 著者 |
Yamada S, Hotta K, Yamamoto TS, Ueno N, Satoh N, Takahashi H.
|
| タイトル |
Interaction of notochord-derived fibrinogen-like protein with Notch regulates the patterning of the central nervous system of Ciona intestinalis embryos.
|
| ジャーナル |
Dev Biol
|
| Abstract |
The midline organ the notochord and its overlying dorsal neural tube are the most prominent features of the chordate body plan. Although the molecular mechanisms involved in the formation of the central nervous system (CNS) have been studied extensively in vertebrate embryos, none of the genes that are expressed exclusively in notochord cells has been shown to function in this process. Here, we report a gene in the urochordate Ciona intestinalis encoding a fibrinogen-like protein that plays a pivotal role in the notochord-dependent positioning of neuronal cells. While this gene (Ci-fibrn) is expressed exclusively in notochord cells, its protein product is not confined to these cells but is distributed underneath the CNS as fibril-like protrusions. We demonstrated that Ci-fibrn interacts physically and functionally with Ci-Notch that is expressed in the central nervous system, and that the correct distribution of Ci-fibrn protein is dependent on Notch signaling. Disturbance of the Ci-fibrn distribution caused an abnormal positioning of neuronal cells and an abnormal track of axon extension. Therefore, it is highly likely that the interaction between the notochord-based fibrinogen-like protein and the neural tube-based Notch signaling plays an essential role in the proper patterning of CNS.
|
| 巻・号 |
328(1)
|
| ページ |
1-12
|
| 公開日 |
2009-4-1
|
| DOI |
10.1016/j.ydbio.2008.12.037
|
| PII |
S0012-1606(08)01468-1
|
| PMID |
19171129
|
| MeSH |
Amino Acid Motifs
Amino Acid Sequence
Animals
Body Patterning / genetics
Central Nervous System / metabolism*
Ciona intestinalis / embryology*
Ciona intestinalis / genetics
Ciona intestinalis / metabolism*
Conserved Sequence
Embryo, Nonmammalian / metabolism
Fibrinogen / genetics
Fibrinogen / metabolism
Gene Expression Regulation, Developmental
Green Fluorescent Proteins / metabolism
Models, Biological
Molecular Sequence Data
Mutation
Notochord / embryology
Notochord / metabolism
Protein Structure, Tertiary
Proteins / genetics
Proteins / metabolism*
Receptor, Notch1 / metabolism*
Recombinant Fusion Proteins / metabolism
Sequence Homology, Amino Acid
|
| IF |
2.896
|
| 引用数 |
9
|
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
|
| リソース情報 |
| カタユウレイボヤ・(ニッポンウミシダ) |
|
