RRC ID 39312
著者 Yamamoto S, Mogi M, Kinpara K, Ishihara Y, Ueda N, Amano K, Nishihara T, Noguchi T, Togari A.
タイトル Anti-proliferative capsular-like polysaccharide antigen from Actinobacillus actinomycetemcomitans induces apoptotic cell death in mouse osteoblastic MC3T3-E1 cells.
ジャーナル J Dent Res
Abstract Actinobacillus actinomycetemcomitans (A. actinomycetemcomitans) has been implicated in the etiology of localized juvenile periodontitis (LJP), and produces a multiplicity of tissue-damaging products. Among those products, the capsular-like polysaccharide antigen (CPA) from A. actinomycetemcomitans is a potent mediator of bone resorption. In fact, this CPA (serotype b) is known to promote osteoclast-like cell formation via interleukin (IL)-1alpha production in mouse marrow cultures. Although osteoblasts complete bone formation, there are few reports focusing on the effect of CPA in bone-forming activity of osteoblasts in inflammatory disease sites. We hypothesized that CPA plays a mediating role in osteoblastic cells. Therefore, the purpose of this study was to examine the effect of CPA from A. actinomycetemcomitans on the mouse osteoblastic cell line MC3T3-E1 and human osteosarcoma SaOS-2 cells. A. actinomycetemcomitans serotype c resulted in a potent dose-dependent inhibition of cell proliferation of both cell lines. Characterization of the antiproliferative activity in the CPA demonstrated that it was not cytotoxic for MC3T3-E1. A 20-hour incubation with CPA-c resulted in a significant increase in apoptotic cell death in the cells, as evaluated by both cellular DNA fragmentation ELISA and FACS analysis. In contrast to the results obtained with a cytokine mixture (tumor necrosis factor-alpha, IL-1beta, and interferon-gamma), no inducible nitric oxide (NO) synthase gene expression or NO release could be detected in MC3T3-E1 after incubation with CPA-c. Further, both CPA-b and -c caused potent induction of apoptosis-related modifiers, e.g., Fas mRNA, whereas bcl-2 mRNA levels were unchanged. Therefore, this study has shown that CPA from A. actinomycetemcomitans contains a potent antiproliferative polysaccharide whose activity is associated with apoptotic cell death in MC3T3-E1, and that CPA per se is an inducer of apoptosis mediated by the Fas system but not by NO.
巻・号 78(6)
ページ 1230-7
公開日 1999-6-1
DOI 10.1177/00220345990780060601
PMID 10371246
MeSH Aggregatibacter actinomycetemcomitans / immunology* Animals Antigens, Bacterial / immunology* Apoptosis* Bacterial Capsules / immunology* Cell Division / drug effects Cell Line DNA Fragmentation Enzyme-Linked Immunosorbent Assay Flow Cytometry Gene Expression Regulation, Enzymologic Humans Inflammation Mediators / immunology Interferon-gamma / immunology Interleukin-1 / immunology Mice Nitric Oxide / metabolism Nitric Oxide Synthase / genetics Osteoblasts / immunology Osteoblasts / pathology* Osteosarcoma / immunology Osteosarcoma / pathology Polysaccharides, Bacterial / immunology* Proto-Oncogene Proteins c-bcl-2 / genetics RNA, Messenger / genetics Tumor Cells, Cultured Tumor Necrosis Factor-alpha / immunology Up-Regulation fas Receptor / genetics
IF 4.914
引用数 25
WOS 分野 DENTISTRY, ORAL SURGERY & MEDICINE
リソース情報
ヒト・動物細胞 Saos-2(RCB0428)