論文 - 詳細
| RRC ID | 39398 |
|---|---|
| 著者 | Katsuura M, Hosono-Sakuma Y, Wagatsuma M, Yanagisawa S, Okazaki M, Kimura M. |
| タイトル | The NH2-terminal region of the active domain of sonic hedgehog is necessary for its signal transduction. |
| ジャーナル | FEBS Lett |
| Abstract |
The NH2-terminal domain of sonic hedgehog (residue 25-198) was expressed in both yeast and animal cells. The yeast-derived NH2-terminal domain of sonic hedgehog was less active by far than the animal cell-derived counterpart. The yeast-derived NH2-terminal domain of sonic hedgehog lacked 10 amino acids from the NH2-terminus. This cleavage of the yeast-derived NH2-terminal domain of sonic hedgehog might due to Kex 2. In contrast, a mutant yeast-derived NH2-terminal domain of sonic hedgehog (Lys-33 to Thr) retained its NH2-terminus and its activity was comparable to that of the animal cell-derived NH2-terminal domain of sonic hedgehog. The NH2-terminal deleted NH2-terminal domain of sonic hedgehog completely lost its activity, nevertheless it inhibited the alkaline phosphatase activity induced by the animal cell-derived NH2-terminal domain of sonic hedgehog in a dose-dependent manner. These data suggest that the NH2-terminal deleted NH2-terminal domain of sonic hedgehog retains a receptor-binding ability and that the NH2-terminal peptide of the NH2-terminal domain of sonic hedgehog is necessary for its signal transduction. |
| 巻・号 | 447(2-3) |
| ページ | 325-8 |
| 公開日 | 1999-3-26 |
| DOI | 10.1016/s0014-5793(99)00300-2 |
| PII | S0014-5793(99)00300-2 |
| PMID | 10214971 |
| MeSH | Alkaline Phosphatase / antagonists & inhibitors Amino Acid Substitution Animals Base Sequence Binding Sites / genetics DNA Primers / genetics Gene Expression Hedgehog Proteins In Vitro Techniques L Cells Mice Proteins / chemistry* Proteins / genetics Proteins / metabolism* Recombinant Proteins / chemistry Recombinant Proteins / genetics Recombinant Proteins / metabolism Saccharomyces cerevisiae / genetics Sequence Deletion Signal Transduction Trans-Activators* |
| IF | 3.057 |
| 引用数 | 9 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | L929 |