Reference - Detail
| RRC ID | 39467 |
|---|---|
| Author | Ishida M, Ohbayashi N, Fukuda M. |
| Title | Rab1A regulates anterograde melanosome transport by recruiting kinesin-1 to melanosomes through interaction with SKIP. |
| Journal | Sci Rep |
| Abstract |
Melanosomes are lysosome-related organelles in melanocytes that are transported from the perinucleus to the cell periphery by coordination between bidirectional (anterograde and retrograde) microtubule-dependent transport and unidirectional actin-dependent transport. Although the molecular machineries that mediate retrograde transport and actin-dependent transport have already been identified, little is known about the anterograde transport complex on microtubules in mammalian cells. Here we discovered that small GTPase Rab1A on melanosomes recruits SKIP/PLEKHM2 as a Rab1A-specific effector and that Rab1A, SKIP, and a kinesin-1/(Kif5b+KLC2) motor form a transport complex that mediates anterograde melanosome transport in melanocytes. Interestingly, Arl8, Arf-like small GTPase that also interacts with SKIP, is specifically localized at lysosomes and regulates their anterograde transport in melanocytes. Our findings suggest that the anterograde microtubule-dependent transport of melanosomes and lysosomes are differently regulated by independent cargo receptors, i.e., Rab1A and Arl8, respectively, but that a SKIP-kinesin-1 mechanism is responsible for the transport of both. |
| Volume | 5 |
| Pages | 8238 |
| Published | 2015-2-4 |
| DOI | 10.1038/srep08238 |
| PII | srep08238 |
| PMID | 25649263 |
| PMC | PMC4316160 |
| MeSH | Adaptor Proteins, Signal Transducing / metabolism* Animals Biological Transport Gene Knockdown Techniques Humans Kinesins / chemistry Kinesins / genetics Kinesins / metabolism* Melanocytes / metabolism Melanosomes / metabolism* Multiprotein Complexes / metabolism Protein Binding Protein Interaction Domains and Motifs Protein Interaction Mapping Two-Hybrid System Techniques rab1 GTP-Binding Proteins / metabolism* |
| IF | 3.998 |
| Times Cited | 15 |
| WOS Category | CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | pEGFP-C1-SKIP/PLEKHM2 (RDB13232) pmStr-C1-SKIP/PLEKHM2 (RDB13233) pmStr-N1-Arl8b (RDB13234) pmStr-N1-Arl8b (Q75L) (RDB13235) pmStr-N1-mouse Arl8b (RDB15388). |