| RRC ID |
39472
|
| 著者 |
Akiyama H, Hiraki Y, Noda M, Shigeno C, Ito H, Nakamura T.
|
| タイトル |
Molecular cloning and biological activity of a novel Ha-Ras suppressor gene predominantly expressed in skeletal muscle, heart, brain, and bone marrow by differential display using clonal mouse EC cells, ATDC5.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
We cloned a cDNA encoding a novel mouse protein, named A-C1, by differential display between two mouse cell lines: embryonic fibroblast C3H10T1/2 and chondrogenic ATDC5. The deduced amino acid sequence of A-C1 consists of 167 amino acids and shows 46% identity with that of a ras-responsive gene, rat Ha-rev107. Northern blot analysis showed a distinct hybridization band of 3.2 kilobases. Expression of A-C1 mRNA was detected in undifferentiated ATDC5 cells and myoblastic C2C12 cells, while none of C3H10T1/2 cells, NIH3T3 fibroblasts, Balb/c 3T3 fibroblasts, osteoblastic MC3T3-E1 cells, and ST2 bone marrow stromal cells expressed A-C1 mRNA in vitro. Moreover, A-C1 mRNA was expressed in skeletal muscle, heart, brain, and bone marrow in adult mice. By in situ hybridization, A-C1 gene expression was localized in hippocampus as well as bone marrow cells. By immunocytochemistry, A-C1 protein was detected in the cytoplasm as well as perinuclear region of the cells. Transfection of A-C1 cDNA into Ha-ras-transformed NIH3T3 cell line caused increase in the number of flat colonies and inhibition of cell growth. Our data indicate that A-C1 is expressed in some specific tissues in vivo and modulates Ha-ras-mediated signaling pathway.
|
| 巻・号 |
274(45)
|
| ページ |
32192-7
|
| 公開日 |
1999-11-5
|
| DOI |
10.1074/jbc.274.45.32192
|
| PII |
S0021-9258(19)51544-7
|
| PMID |
10542256
|
| MeSH |
3T3 Cells
Amino Acid Sequence
Animals
Base Sequence
Bone Marrow / metabolism*
Brain / metabolism*
Cell Line
Clone Cells
Cloning, Molecular
Gene Library
Genes, ras*
In Situ Hybridization
Male
Mice
Mice, Inbred C3H
Molecular Sequence Data
Muscle, Skeletal / metabolism*
Myocardium / metabolism*
Peptide Library
RNA, Messenger / metabolism
Rats
Transfection
|
| IF |
4.238
|
| 引用数 |
44
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
10T1/2(RCB0247)
BALB/3T3 clone A31(RCB0005)
NIH3T3
C2C12(RCB0987) |
