論文 - 詳細
| RRC ID | 39472 |
|---|---|
| 著者 | Akiyama H, Hiraki Y, Noda M, Shigeno C, Ito H, Nakamura T. |
| タイトル | Molecular cloning and biological activity of a novel Ha-Ras suppressor gene predominantly expressed in skeletal muscle, heart, brain, and bone marrow by differential display using clonal mouse EC cells, ATDC5. |
| ジャーナル | J Biol Chem |
| Abstract |
We cloned a cDNA encoding a novel mouse protein, named A-C1, by differential display between two mouse cell lines: embryonic fibroblast C3H10T1/2 and chondrogenic ATDC5. The deduced amino acid sequence of A-C1 consists of 167 amino acids and shows 46% identity with that of a ras-responsive gene, rat Ha-rev107. Northern blot analysis showed a distinct hybridization band of 3.2 kilobases. Expression of A-C1 mRNA was detected in undifferentiated ATDC5 cells and myoblastic C2C12 cells, while none of C3H10T1/2 cells, NIH3T3 fibroblasts, Balb/c 3T3 fibroblasts, osteoblastic MC3T3-E1 cells, and ST2 bone marrow stromal cells expressed A-C1 mRNA in vitro. Moreover, A-C1 mRNA was expressed in skeletal muscle, heart, brain, and bone marrow in adult mice. By in situ hybridization, A-C1 gene expression was localized in hippocampus as well as bone marrow cells. By immunocytochemistry, A-C1 protein was detected in the cytoplasm as well as perinuclear region of the cells. Transfection of A-C1 cDNA into Ha-ras-transformed NIH3T3 cell line caused increase in the number of flat colonies and inhibition of cell growth. Our data indicate that A-C1 is expressed in some specific tissues in vivo and modulates Ha-ras-mediated signaling pathway. |
| 巻・号 | 274(45) |
| ページ | 32192-7 |
| 公開日 | 1999-11-5 |
| DOI | 10.1074/jbc.274.45.32192 |
| PII | S0021-9258(19)51544-7 |
| PMID | 10542256 |
| MeSH | 3T3 Cells Amino Acid Sequence Animals Base Sequence Bone Marrow / metabolism* Brain / metabolism* Cell Line Clone Cells Cloning, Molecular Gene Library Genes, ras* In Situ Hybridization Male Mice Mice, Inbred C3H Molecular Sequence Data Muscle, Skeletal / metabolism* Myocardium / metabolism* Peptide Library RNA, Messenger / metabolism Rats Transfection |
| IF | 4.238 |
| 引用数 | 44 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 10T1/2(RCB0247) BALB/3T3 clone A31(RCB0005) NIH3T3-3(RCB0150) C2C12(RCB0987) |