| RRC ID |
39481
|
| 著者 |
Koike E, Kobayashi T, Murakami M, McWilliam AS, Holt PG.
|
| タイトル |
Mechanisms of ozone-induced inhibitory effect of bronchoalveolar lavage fluid on alveolar macrophage-mediated immunosuppressive activity in rats.
|
| ジャーナル |
J Leukoc Biol
|
| Abstract |
Resident alveolar macrophages (AM) play an important immunomodulatory role via suppression of lymphocyte proliferation, and nitric oxide (NO) plays a crucial role in this immunosuppression of AM. Our previous report suggested that during ozone (O3)-induced lung inflammation, bronchoalveolar lavage fluid (BALF) inhibited AM-mediated immunosuppression and concanavalin A (Con A)-induced proliferation of lymph node cells (LNC) [E. Koike et al. (1998) Toxicol. Sci. 41, 217-223]. In these studies, we investigated the mechanisms of the inhibition of BALF from O3-exposed rats (O3-BALF). We investigated whether BALF might affect (1) the interferon-gamma (IFN-gamma) production by Con A-stimulated LNC and IFN-gamma-induced NO production by AM, and (2) the interleukin (IL)-2 production by Con A-stimulated LNC and IL-2-induced LNC proliferation. These results demonstrated that O3-BALF inhibited IFN-gamma production by Con A-stimulated LNC, IFN-gamma-induced NO production by AM, and IL-2-induced LNC proliferation. In addition, the major inhibitory factor against AM-mediated immunosuppression in O3-BALF may be a protein of greater than 10 kDa.
|
| 巻・号 |
66(1)
|
| ページ |
75-82
|
| 公開日 |
1999-7-1
|
| DOI |
10.1002/jlb.66.1.75
|
| PMID |
10410992
|
| MeSH |
Animals
Bronchoalveolar Lavage Fluid
Cell Division
Concanavalin A / pharmacology
Immune Tolerance / immunology*
Interferon-gamma / immunology
Lymph Nodes / cytology
Macrophages, Alveolar / immunology*
Male
Mitogens / pharmacology
Neutralization Tests
Nitric Oxide / metabolism
Ozone / immunology*
Rats
Rats, Wistar
|
| IF |
3.757
|
| 引用数 |
7
|
|
WOS 分野
|
HEMATOLOGY
IMMUNOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
