Reference - Detail
| RRC ID | 39491 |
|---|---|
| Author | Suzuki J, Kaziro Y, Koide H. |
| Title | Positive regulation of skeletal myogenesis by R-Ras. |
| Journal | Oncogene |
| Abstract |
Most of the proteins in the Ras-family proteins, including Ras, Rap and TC21, have been reported to be strong inhibitors of skeletal myogenesis. Here we show that R-Ras, another member of this family, promotes terminal differentiation of C2C12 skeletal myoblasts. In contrast to Ras, which induced a markedly transformed phenotype of C2C12 cells, an activated mutant of R-Ras (R-RasQ87L) did not exhibit any inhibitory effect on the differentiation of C2C12 cells, but enhanced the formation of multinucleated myotubes. Although R-RasQ87L showed little effect on induction of two muscle-specific proteins, creatine kinase and myogenin, it prevented cell death during myoblast differentiation, probably through Akt activation and Bcl-xL induction. Motility of C2C12 cells, which may be involved in fusion of myoblasts, was also stimulated by R-RasQ87L. Furthermore, we observed a transient activation of endogenous R-Ras during differentiation of C2C12 cells. The ectopic expression of R-Ras GAP inhibited the differentiation. These results suggest that R-Ras has a positive effect on the terminal differentiation of myoblasts and may be involved in the program of skeletal myogenesis. |
| Volume | 19(9) |
| Pages | 1138-46 |
| Published | 2000-2-24 |
| DOI | 10.1038/sj.onc.1203402 |
| PMID | 10713701 |
| MeSH | Animals Cell Death Cell Differentiation / genetics Cell Differentiation / physiology Cell Line Cell Movement Creatine Kinase / biosynthesis Enzyme Induction GTP Phosphohydrolases / genetics GTP Phosphohydrolases / physiology* Gene Expression Regulation Mice Muscle, Skeletal / cytology* Muscle, Skeletal / enzymology Muscle, Skeletal / metabolism Muscle, Skeletal / physiology* Mutation Myogenin / biosynthesis Oncogene Proteins / genetics Oncogene Proteins / physiology* Transfection ras Proteins / genetics ras Proteins / physiology* |
| IF | 7.971 |
| Times Cited | 15 |
| WOS Category | ONCOLOGY GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | C2C12(RCB0987) |