RRC ID 3954
Author Ohshima N, Yamashita S, Takahashi N, Kuroishi C, Shiro Y, Takio K.
Title Escherichia coli cytosolic glycerophosphodiester phosphodiesterase (UgpQ) requires Mg2+, Co2+, or Mn2+ for its enzyme activity.
Journal J Bacteriol
Abstract Escherichia coli cytosolic glycerophosphodiester phosphodiesterase, UgpQ, functions in the absence of other proteins encoded by the ugp operon and requires Mg2+, Mn2+, or Co2+, in contrast to Ca2+-dependent periplasmic glycerophosphodiester phosphodiesterase, GlpQ. UgpQ has broad substrate specificity toward various glycerophosphodiesters, producing sn-glycerol-3-phosphate and the corresponding alcohols. UgpQ accumulates under conditions of phosphate starvation, suggesting that it allows the utilization of glycerophosphodiesters as a source of phosphate. These results clarify how E. coli utilizes glycerophosphodiesters using two homologous enzymes, UgpQ and GlpQ.
Volume 190(4)
Pages 1219-23
Published 2008-2-1
DOI 10.1128/JB.01223-07
PII JB.01223-07
PMID 18083802
PMC PMC2238210
MeSH Bacterial Proteins / genetics Bacterial Proteins / metabolism Cobalt / metabolism Cytosol / metabolism Electrophoresis, Polyacrylamide Gel Enzyme Activation Escherichia coli / enzymology* Escherichia coli / genetics Escherichia coli / growth & development Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Gene Expression Regulation, Bacterial Immunoblotting Magnesium / metabolism Manganese / metabolism Metals / metabolism* Phosphates / metabolism Phosphoric Diester Hydrolases / genetics Phosphoric Diester Hydrolases / metabolism* Protons Recombinant Proteins / metabolism Substrate Specificity
IF 3.234
Times Cited 31
WOS Category MICROBIOLOGY
Resource
Prokaryotes E. coli JW3414 ME9012