論文 - 詳細
| RRC ID | 39540 |
|---|---|
| 著者 | Mashima J, Nagahama M, Hatsuzawa K, Tani K, Horigome T, Yamamoto A, Tagaya M. |
| タイトル | N-ethylmaleimide-sensitive factor is associated with the nuclear envelope. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
N-Ethylmaleimide-sensitive factor (NSF) is an ATPase involved in many membrane fusion events within the exocytic and endocytotic pathways. In the present study we showed that NSF is associated with the nuclear envelope. Golgi-associated NSF was released from membranes upon incubation with Mg(2+)-ATP, reflecting the disassembly of a complex consisting of NSF, soluble NSF attachment proteins (SNAPs), and SNAP receptors (SNAREs). In contrast nuclear envelope-associated NSF in interphase cells was not released by the same treatment. During mitosis, however, it was released from nuclear membranes by Mg(2+)-ATP. These results suggest that the binding mode of nuclear membrane-associated NSF changes during the cell cycle. |
| 巻・号 | 274(2) |
| ページ | 559-64 |
| 公開日 | 2000-8-2 |
| DOI | 10.1006/bbrc.2000.3162 |
| PII | S0006-291X(00)93162-0 |
| PMID | 10913377 |
| MeSH | Adenosine Triphosphate / pharmacology Animals Carrier Proteins / analysis Carrier Proteins / metabolism* Cell Cycle Cell Division Cell Fractionation Cell Nucleus / chemistry Cell Nucleus / metabolism Cytoplasm / metabolism Immunoblotting Interphase Lamins Mannosidases / analysis Mitosis / drug effects N-Ethylmaleimide-Sensitive Proteins Nuclear Envelope / metabolism* Nuclear Envelope / ultrastructure Nuclear Proteins / metabolism PC12 Cells Rats Vesicular Transport Proteins* |
| IF | 2.985 |
| 引用数 | 3 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | PC-12(RCB0009) NRK(RCB0043) |