論文 - 詳細
| RRC ID | 3954 |
|---|---|
| 著者 | Ohshima N, Yamashita S, Takahashi N, Kuroishi C, Shiro Y, Takio K. |
| タイトル | Escherichia coli cytosolic glycerophosphodiester phosphodiesterase (UgpQ) requires Mg2+, Co2+, or Mn2+ for its enzyme activity. |
| ジャーナル | J Bacteriol |
| Abstract |
Escherichia coli cytosolic glycerophosphodiester phosphodiesterase, UgpQ, functions in the absence of other proteins encoded by the ugp operon and requires Mg2+, Mn2+, or Co2+, in contrast to Ca2+-dependent periplasmic glycerophosphodiester phosphodiesterase, GlpQ. UgpQ has broad substrate specificity toward various glycerophosphodiesters, producing sn-glycerol-3-phosphate and the corresponding alcohols. UgpQ accumulates under conditions of phosphate starvation, suggesting that it allows the utilization of glycerophosphodiesters as a source of phosphate. These results clarify how E. coli utilizes glycerophosphodiesters using two homologous enzymes, UgpQ and GlpQ. |
| 巻・号 | 190(4) |
| ページ | 1219-23 |
| 公開日 | 2008-2-1 |
| DOI | 10.1128/JB.01223-07 |
| PII | JB.01223-07 |
| PMID | 18083802 |
| PMC | PMC2238210 |
| MeSH | Bacterial Proteins / genetics Bacterial Proteins / metabolism Cobalt / metabolism Cytosol / metabolism Electrophoresis, Polyacrylamide Gel Enzyme Activation Escherichia coli / enzymology* Escherichia coli / genetics Escherichia coli / growth & development Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Gene Expression Regulation, Bacterial Immunoblotting Magnesium / metabolism Manganese / metabolism Metals / metabolism* Phosphates / metabolism Phosphoric Diester Hydrolases / genetics Phosphoric Diester Hydrolases / metabolism* Protons Recombinant Proteins / metabolism Substrate Specificity |
| IF | 3.006 |
| 引用数 | 31 |
| WOS 分野 | MICROBIOLOGY |
| リソース情報 | |
| 原核生物(大腸菌) | JW3414 ME9012 |