RRC ID 39550
Author Aoki N, Kimura S, Takiyama Y, Atsuta Y, Abe A, Sato K, Katagiri M.
Title The role of the DAP12 signal in mouse myeloid differentiation.
Journal J Immunol
Abstract DAP12 is a recently cloned, immunoreceptor tyrosine-based activation motif-bearing transmembrane adapter molecule that is associated with the NK-activating receptors. Previous reports showed that the DAP12 message could be detected not only in NK cells but also in granulocytes, monocytes, dendritic cells, and macrophages. In this study we found a significant level of DAP12 protein expression in macrophage-related cell lines and organs. Additionally, we observed increased expression of DAP12 after LPS-induced differentiation of M1 cells into macrophages. To examine the role of DAP12 in the myeloid cell lineage, we established M1 FLAG-DAP12 transfectants (FDAP-M1) and demonstrated the marked morphological changes in FDAP-M1 cells caused by signaling through DAP12. Cell surface phenotypic analysis showed up-regulation of macrophage markers CD11b, 2.4G2, and adhesion molecule B7-2. Additionally, after stimulation through DAP12, phosphorylated FLAG -DAP12 could be immunoprecipitated using anti-phosphotyrosine mAbs. Collectively, these findings indicate that direct DAP12 signaling has an important role in macrophage differentiation.
Volume 165(7)
Pages 3790-6
Published 2000-10-1
DOI 10.4049/jimmunol.165.7.3790
PMID 11034384
MeSH Adaptor Proteins, Signal Transducing Animals Antibodies, Monoclonal / metabolism Cell Differentiation / genetics Cell Differentiation / immunology Cell Line Cell Membrane / immunology Cell Membrane / metabolism Immunophenotyping Leukemia P388 Lipopolysaccharides / pharmacology Macrophage Activation / immunology Macrophages / immunology Macrophages / metabolism Membrane Proteins Mice Mice, Inbred BALB C Mice, Inbred C3H Mice, Inbred DBA Myeloid Cells / cytology* Myeloid Cells / immunology* Myeloid Cells / metabolism Oligopeptides / biosynthesis Oligopeptides / genetics Oligopeptides / immunology Organ Specificity / immunology Peptides / genetics Peptides / immunology Phosphorylation Receptors, Immunologic / biosynthesis Receptors, Immunologic / genetics Receptors, Immunologic / metabolism Receptors, Immunologic / physiology* Recombinant Proteins / biosynthesis Recombinant Proteins / immunology Signal Transduction / genetics Signal Transduction / immunology* Transfection Tumor Cells, Cultured
IF 4.886
Times Cited 34
Human and Animal Cells