| RRC ID |
39555
|
| 著者 |
Kim HS, Yamashita S, Akao T, Saitoh H, Higuchi K, Park YS, Mizuki E, Ohba M.
|
| タイトル |
In vitro cytotoxicity of non-cyt inclusion proteins of a Bacillus thuringiensis isolate against human cells, including cancer cells.
|
| ジャーナル |
J Appl Microbiol
|
| Abstract |
A soil isolate designated 90-F-45-14, belonging to Bacillus thuringiensis serovar dakota (H15), was examined for characterization of in vitro cytotoxicity, associated with parasporal inclusion proteins, against human cells. When activated with proteolytic processing, inclusion proteins of the isolate 90-F-45-14 exhibited a moderate cytotoxicity against the human uterus cervix cancer cells (HeLa) with an EC(50) value of 60.8 microg ml(-1), while showing extremely high activities on the human leukaemic T cells (MOLT-4) and the normal T cells with EC(50) values of 0.27 and 0.20 microg ml(-1), respectively. Anti-leukaemic cell activity of the 90-F-45-14 proteins was eight to nine times greater than that of the B. thuringiensis serovar israelensis proteins containing the Cyt1 protein, a broad-spectrum cytolysin. The cytopathy by the 90-F-45-14 proteins was characterized by marked cell-ballooning, while the israelensis proteins induced early breakdown of the cells due to cytolysis. Inclusions of the isolate consisted of five major polypeptides of 170, 103, 73, 40 and 32 kDa. A 100% homology was observed in the sequence of 15 N-terminal amino acids between the proteins of 170 and 103 kDa. There was no N-terminal sequence homology between 90-F-45-14 proteins and the existing Cry/Cyt proteins of B. thuringiensis. Proteolytic processing by proteinase K yielded several proteins with molecular masses ranging from 40 to 28 kDa.
|
| 巻・号 |
89(1)
|
| ページ |
16-23
|
| 公開日 |
2000-7-1
|
| DOI |
10.1046/j.1365-2672.2000.01087.x
|
| PMID |
10945774
|
| MeSH |
Amino Acid Sequence
Bacillus thuringiensis* / ultrastructure
Bacterial Proteins / chemistry
Bacterial Proteins / toxicity*
Cell Death*
Cells, Cultured
Endopeptidase K / metabolism
HeLa Cells
Humans
Inclusion Bodies / chemistry
Inclusion Bodies / ultrastructure
Molecular Weight
Spores, Bacterial
T-Lymphocytes
Tumor Cells, Cultured
|
| IF |
3.066
|
| 引用数 |
24
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MICROBIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
HeLa(RCB0007)
MOLT-4(RCB0206) |
