| RRC ID |
39568
|
| 著者 |
Dohmoto K, Hojo S, Fujita J, Ueda Y, Bandoh S, Yamaji Y, Ohtsuki Y, Dobashi N, Takahara J.
|
| タイトル |
Mechanisms of the release of CYFRA21-1 in human lung cancer cell lines.
|
| ジャーナル |
Lung Cancer
|
| Abstract |
The CYFRA 21-1 assay which detects cytokeratin 19 (CK19) fragment is widely used as a tumor marker for lung cancer. However, the reason why some lung cancer cell lines release CK19 fragment in culture supernatants and others do not, remains unclear. It was hypothesized that the release of CK19 fragment may be elucidated by the expression of mRNA for CK19. In order to prove this, the mRNA for CK19 was quantitatively evaluated by the competitive reverse transcriptase-polymerase chain reaction (competitive RT-PCR). The level of CYFRA 21-1 in the culture supernatant was measured by an immunoradiometric assay. CK19 protein synthesis was evaluated by a Western blotting and immunohistochemistry. Fourteen lung cancer cell lines were evaluated, and the amount of mRNA correlated well with the level of CYFRA 21-1 in culture supernatants. Analysis of genomic DNA for CK19 demonstrated that three cell lines which could not produce CYFRA 21-1, conjectured that some abnormalities in exon 1 or the 5'-region upstream from exon 1. In conclusion, it was demonstrated that the release of CK19 fragment was closely related to the expression of mRNA for CK19, and the possibility that genomic change of CK19 DNA down-regulated the expression of mRNA for CK19 was suggested.
|
| 巻・号 |
30(1)
|
| ページ |
55-63
|
| 公開日 |
2000-10-1
|
| DOI |
10.1016/s0169-5002(00)00125-2
|
| PII |
S0169-5002(00)00125-2
|
| PMID |
11008010
|
| MeSH |
Antigens, Neoplasm / analysis*
Blotting, Western
Carcinoma, Non-Small-Cell Lung / pathology*
Carcinoma, Small Cell / pathology*
DNA, Neoplasm
Down-Regulation
Gene Expression Profiling
Humans
Immunohistochemistry
Keratin-19
Keratins / analysis*
Keratins / metabolism*
Lung Neoplasms / pathology*
RNA, Messenger / analysis
Reverse Transcriptase Polymerase Chain Reaction
Tumor Cells, Cultured
|
| IF |
4.702
|
| 引用数 |
12
|
|
WOS 分野
|
RESPIRATORY SYSTEM
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
