RRC ID 39586
Author Fukuda M, Kanno E, Saegusa C, Ogata Y, Kuroda TS.
Title Slp4-a/granuphilin-a regulates dense-core vesicle exocytosis in PC12 cells.
Journal J Biol Chem
Abstract Synaptotagmin-like protein 4-a (Slp4-a)/granuphilin-a was originally identified as a protein specifically associated with insulin-containing vesicles in pancreatic beta-cells (Wang, J., Takeuchi, T., Yokota, H., and Izumi, T. (1999) J. Biol. Chem. 274, 28542-28548). Previously, we showed that the N-terminal Slp homology domain of Slp4-a interacts with the GTP-bound form of Rab3A, Rab8, and Rab27A both in vitro and in intact cells (Kuroda, T. S., Fukuda, M., Ariga, H., and Mikoshiba, K. (2002) J. Biol. Chem. 277, 9212-9218). How Slp4-a.Rab complex controls regulated secretion, and which Rab isoforms dominantly interact with Slp4-a in vivo, however, have remained unknown. In this study, we showed by immunocytochemistry and subcellular fractionation that three Rabs, Rab3A, Rab8, and Rab27A, and Slp4-a are endogenously expressed in neuroendocrine PC12 cells and localized on dense-core vesicles, and we discovered that the Slp4-a.Rab8 and Slp4-a.Rab27A complexes, but not Slp4-a.Rab3A complexes, are formed on dense-core vesicles in PC12 cells, although the majority of Rab8 is present in the cell body and is free of Slp4-a. We further showed that expression of Rab27A, but not of Rab8, promotes high KCl-dependent secretion of neuropeptide Y (NPY) in PC12 cells, whereas expression of Slp4-a, but not of an Slp4-a mutant incapable of Rab27A binding, inhibits NPY secretion in PC12 cells. In contrast, expression of Slp3-a, but not of Slp3-b lacking an N-terminal Rab27A-binding domain, promotes NPY secretion. These findings suggest that the Slp family controls regulated dense-core vesicle exocytosis via binding to Rab27A.
Volume 277(42)
Pages 39673-8
Published 2002-10-18
DOI 10.1074/jbc.M205349200
PII M205349200
PMID 12176990
MeSH Animals Carrier Proteins / chemistry* Carrier Proteins / physiology* DNA, Complementary / metabolism Exocytosis Genetic Vectors Glutathione Transferase / metabolism Immunoblotting Mice PC12 Cells Protein Binding Protein Structure, Tertiary Rats Recombinant Fusion Proteins / metabolism Transfection Vesicular Transport Proteins rab GTP-Binding Proteins / metabolism rab27 GTP-Binding Proteins rab3A GTP-Binding Protein / metabolism
IF 4.238
Times Cited 37
DNA material Slp4-a (RDB034219 Rab27A (RDB03423).