RRC ID 396
著者 Kawakami A, Nojima Y, Toyoda A, Takahoko M, Satoh M, Tanaka H, Wada H, Masai I, Terasaki H, Sakaki Y, Takeda H, Okamoto H.
タイトル The zebrafish-secreted matrix protein you/scube2 is implicated in long-range regulation of hedgehog signaling.
ジャーナル Curr Biol
Abstract The Hedgehog (Hh) signal plays a pivotal role in induction of ventral neuronal and muscle cell types around the midline during vertebrate development [1]. We report that the gene disrupted in zebrafish you mutants, in which Hh signaling is impaired, encodes the secreted matrix protein Scube2. Consistently, epistasis analyses suggested that Scube2 functions upstream of Hh ligands or through a parallel pathway. In addition, overexpression analyses suggested that Scube2 is an essential, but a permissive, mediator of Hh signaling in zebrafish embryos. Surprisingly, the you gene is expressed in the dorsal neural tube, raising the possibility that Scube2 could indirectly act via a long-range regulator of Hh signaling. The dorsal Bmps have a long-range and opposing influence on Hh signaling [2-5]. We show that neural plate patterning is affected in you mutants in a way that is consistent with the aberrant long-range action of a Bmp-dependent signal. We further show that Bmp activity can be attenuated by the coexpression of Scube2. Our data support the idea that Scube2 can modulate the long-range action of Bmp-dependent signaling in the neural tube and somites.
巻・号 15(5)
ページ 480-8
公開日 2005-3-8
DOI 10.1016/j.cub.2005.02.018
PII S0960-9822(05)00157-0
PMID 15753045
MeSH Amino Acid Sequence Animals Base Sequence Blotting, Western Body Patterning / physiology* Bone Morphogenetic Proteins / metabolism Central Nervous System / embryology* Chromosome Mapping DNA Primers Epistasis, Genetic Extracellular Matrix Proteins / genetics Extracellular Matrix Proteins / metabolism* Gene Components Genotype Hedgehog Proteins In Situ Hybridization Molecular Sequence Data Mutation / genetics Phenotype* Sequence Alignment Sequence Analysis, DNA Signal Transduction / physiology* Trans-Activators / metabolism* Zebrafish / embryology Zebrafish / metabolism* Zebrafish Proteins / genetics Zebrafish Proteins / metabolism*
IF 9.601
引用数 81
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
ゼブラフィッシュ rw87 RIKEN WT (RW)