RRC ID 39606
Author Michigami T, Shimizu N, Williams PJ, Niewolna M, Dallas SL, Mundy GR, Yoneda T.
Title Cell-cell contact between marrow stromal cells and myeloma cells via VCAM-1 and alpha(4)beta(1)-integrin enhances production of osteoclast-stimulating activity.
Journal Blood
Abstract Myeloma is a unique hematologic malignancy that exclusively homes in the bone marrow and induces massive osteoclastic bone destruction presumably by producing cytokines that promote the differentiation of the hematopoietic progenitors to osteoclasts (osteoclastogenesis). It is recognized that neighboring bone marrow stromal cells influence the expression of the malignant phenotype in myeloma cells. This study examined the role of the interactions between myeloma cells and neighboring stromal cells in the production of osteoclastogenic factors to elucidate the mechanism underlying extensive osteoclastic bone destruction. A murine myeloma cell line 5TGM1, which causes severe osteolysis, expresses alpha(4)beta(1)-integrin and tightly adheres to the mouse marrow stromal cell line ST2, which expresses the vascular cell adhesion molecule-1 (VCAM-1), a ligand for alpha(4)beta(1)-integrin. Co-cultures of 5TGM1 with primary bone marrow cells generated tartrate-resistant acid phosphatase-positive multinucleated bone-resorbing osteoclasts. Co-cultures of 5TGM1 with ST2 showed increased production of bone-resorbing activity and neutralizing antibodies against VCAM-1 or alpha(4)beta(1)-integrin inhibited this. The 5TGM1 cells contacting recombinant VCAM-1 produced increased osteoclastogenic and bone-resorbing activity. The activity was not blocked by the neutralizing antibody to known osteoclastogenic cytokines including interleukin (IL)-1, IL-6, tumor necrosis factor, or parathyroid hormone-related peptide. These data suggest that myeloma cells are responsible for producing osteoclastogenic activity and that establishment of direct contact with marrow stromal cells via alpha(4)beta(1)-integrin/VCAM-1 increases the production of this activity by myeloma cells. They also suggest that the presence of stromal cells may provide a microenvironment that allows exclusive colonization of myeloma cells in the bone marrow. (Blood. 2000;96:1953-1960)
Volume 96(5)
Pages 1953-60
Published 2000-9-1
PII S0006-4971(20)54476-5
PMID 10961900
MeSH Acid Phosphatase / metabolism Animals Antibodies, Monoclonal / immunology Antibodies, Monoclonal / pharmacology Antigens, CD / metabolism Bone Marrow Cells / cytology Bone Marrow Cells / metabolism* Bone Resorption / physiopathology CHO Cells Cell Adhesion / drug effects Cell Communication* Coculture Techniques Cricetinae Culture Media, Conditioned / pharmacology Female Gene Expression Humans Integrin alpha4 Integrin alpha4beta1 Integrins / genetics Integrins / immunology Integrins / metabolism* Isoenzymes / metabolism Mice Mice, Inbred C57BL Multiple Myeloma / metabolism Multiple Myeloma / pathology Neutralization Tests Osteoclasts / cytology Osteoclasts / drug effects Osteoclasts / physiology* Protein Binding RNA, Messenger / genetics RNA, Messenger / metabolism Rats Receptors, Lymphocyte Homing / genetics Receptors, Lymphocyte Homing / immunology Receptors, Lymphocyte Homing / metabolism* Recombinant Proteins / metabolism Solubility Stromal Cells / cytology Stromal Cells / metabolism* Tartrate-Resistant Acid Phosphatase Tumor Cells, Cultured Vascular Cell Adhesion Molecule-1 / genetics Vascular Cell Adhesion Molecule-1 / immunology Vascular Cell Adhesion Molecule-1 / metabolism*
IF 17.794
Times Cited 174
Human and Animal Cells ST2(RCB0224)