Reference - Detail
| RRC ID | 39614 |
|---|---|
| Author | Enomoto H, Enomoto-Iwamoto M, Iwamoto M, Nomura S, Himeno M, Kitamura Y, Kishimoto T, Komori T. |
| Title | Cbfa1 is a positive regulatory factor in chondrocyte maturation. |
| Journal | J Biol Chem |
| Abstract |
Cbfa1 is a transcription factor that belongs to the runt domain gene family. Cbfa1-deficient mice showed a complete lack of bone formation due to the maturational arrest of osteoblasts, demonstrating that Cbfa1 is an essential factor for osteoblast differentiation. Further, chondrocyte maturation was severely disturbed in Cbfa1-deficient mice. In this study, we examined the possibility that Cbfa1 is also involved in the regulation of chondrocyte differentiation. mRNAs for both Cbfa1 isotypes, type I Cbfa1 (Pebp2alphaA/Cbfa1) and type II Cbfa1 (Osf2/Cbfa1 or til-1), which are different in N-terminal domain, were expressed in terminal hypertrophic chondrocytes as well as osteoblasts. In addition, mRNA for type I Cbfa1 was expressed in other hypertrophic chondrocytes and prehypertrophic chondropcytes. In a chondrogenic cell line, ATDC5, the expression of type I Cbfa1 was elevated prior to differentiation to the hypertrophic phenotype, which is characterized by type X collagen expression. Treatment with antisense oligonucleotides for type I Cbfa1 severely reduced type X collagen expression in ATDC5 cells. Retrovirally forced expression of either type I or type II Cbfa1 in chick immature chondrocytes induced type X collagen and MMP13 expression, alkaline phosphatase activity, and extensive cartilage-matrix mineralization. These results indicate that Cbfa1 is an important regulatory factor in chondrocyte maturation. |
| Volume | 275(12) |
| Pages | 8695-702 |
| Published | 2000-3-24 |
| DOI | 10.1074/jbc.275.12.8695 |
| PII | S0021-9258(18)30177-7 |
| PMID | 10722711 |
| MeSH | Animals Cell Differentiation / drug effects Chick Embryo Chondrocytes / cytology* Chondrocytes / metabolism Core Binding Factor Alpha 1 Subunit DNA, Complementary / genetics DNA-Binding Proteins / biosynthesis* DNA-Binding Proteins / classification DNA-Binding Proteins / genetics Hypertrophy Mice Neoplasm Proteins* Oligonucleotides, Antisense / pharmacology Osteoblasts Phenotype RNA, Messenger / analysis Tibia / cytology Transcription Factor AP-2 Transcription Factors / biosynthesis* Transcription Factors / classification Transcription Factors / genetics |
| IF | 4.238 |
| Times Cited | 315 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 32 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | ATDC5(RCB0565) |