論文 - 詳細
| RRC ID | 39630 |
|---|---|
| 著者 | Takeuchi Y, Suzawa M, Fukumoto S, Fujita T. |
| タイトル | Vitamin K(2) inhibits adipogenesis, osteoclastogenesis, and ODF/RANK ligand expression in murine bone marrow cell cultures. |
| ジャーナル | Bone |
| Abstract |
Several lines of evidence suggest that vitamin K has nutritional and pharmacological effects against bone loss. To clarify effects of vitamin K on bone marrow cells, which contains progenitors of both osteoblasts and osteoclasts, we examined mouse bone marrow cell cultures in the presence of vitamin K(1) (K1) and menatetrenone (MK4), a vitamin K(2) with four isoprene units. Treatment with MK4 but not K1 inhibited the formation of adipocytes and stimulated alkaline phosphatase activity, an early differentiation marker of osteoblast. Although nuclear receptor PPARgamma2 plays a pivotal role in adipogenesis, MK4 had no effects on the expression of PPARgamma2 mRNA and PPARgamma2-dependent transcriptional activity. MK4 inhibited the expression of osteoclast differentiation factor (ODF)/RANK ligand and the formation of osteoclast-like cells induced by 1,25-dihydroxyvitamin D(3). These results suggest that MK4 specifically influences differentiation and functions of bone marrow cells to inhibit adipogenesis and osteoclastogenesis. At the expense of adipogenesis, MK4 might stimulate osteoblastogenesis in bone marrow cells. Therefore, MK4 may favor bone metabolism to spare bone mass as a compound that modulates cellular differentiation and functions in bone marrow in addition to as a nutrient factor. |
| 巻・号 | 27(6) |
| ページ | 769-76 |
| 公開日 | 2000-12-1 |
| DOI | 10.1016/s8756-3282(00)00396-3 |
| PII | S8756328200003963 |
| PMID | 11113387 |
| MeSH | Adipocytes / cytology* Adipocytes / drug effects Animals Antineoplastic Agents / pharmacology Bone Marrow Cells / cytology* Bone Marrow Cells / drug effects Bone and Bones / cytology Bone and Bones / metabolism Calcitriol / pharmacology Calcium Channel Agonists / pharmacology Carrier Proteins / genetics* Cell Differentiation / drug effects Cell Differentiation / physiology Cells, Cultured Chromans / pharmacology Gene Expression / drug effects Hemostatics / pharmacology* Male Membrane Glycoproteins / genetics* Mice Osteoclasts / cytology* Osteoclasts / drug effects RANK Ligand RNA, Messenger / analysis Receptor Activator of Nuclear Factor-kappa B Receptors, Cytoplasmic and Nuclear / genetics Thiazoles / pharmacology Thiazolidinediones* Transcription Factors / genetics Troglitazone Vitamin K / analogs & derivatives* Vitamin K / pharmacology* Vitamin K 2 / analogs & derivatives |
| IF | 4.147 |
| 引用数 | 58 |
| WOS 分野 | ENDOCRINOLOGY & METABOLISM |
| リソース情報 | |
| ヒト・動物細胞 | ST2(RCB0224) |