論文 - 詳細
| RRC ID | 39632 |
|---|---|
| 著者 | Chien HH, Lin WL, Cho MI. |
| タイトル | Down-regulation of osteoblastic cell differentiation by epidermal growth factor receptor. |
| ジャーナル | Calcif Tissue Int |
| Abstract |
The role of epidermal growth factor receptors (EGF-R) in osteogenic cell differentiation was investigated using preosteoblastic MC3T3-E1 (MC3T3) cells and osteoblast-like ROS 17/2.8 (ROS) cells. When cultured in the presence of beta-glycerophosphate (GP) and ascorbic acid (AA), MC3T3 cells underwent spontaneous differentiation into osteoblasts which was confirmed as they expressed osteoblast markers such as alkaline phosphatase (ALP), bone sialoprotein (BSP) and osteocalcin (OC). Interestingly, the number of EGF-binding sites decreased during their differentiation into osteoblasts, and the osteogenic protein-1 (OP-1) treatment, which accelerated their differentiation, lowered the number of EGF-binding sites even further. On the other hand, ROS cells with high expression levels of osteoblast markers and no EGF-R, after being transfected with human EGF-R cDNA (EROS cells), expressed numerous EGF-binding sites as well as EGF-R mRNA and protein; in the process, they ceased to express osteoblast markers, indicating their dedifferentiation into osteoprogenitor cells. Both MC3T3 and EROS cells showed increased cell growth in response to EGF, whereas ROS cells did not. These results imply that the EGF/EGF-R system in osteogenic cells has a crucial function in osteoblast phenotype suppression and osteogenic cell proliferation. |
| 巻・号 | 67(2) |
| ページ | 141-50 |
| 公開日 | 2000-8-1 |
| DOI | 10.1007/s00223001128 |
| PII | 10.1007/s00223001128 |
| PMID | 10920219 |
| MeSH | Alkaline Phosphatase / genetics Ascorbic Acid / pharmacology Blotting, Northern Bone Morphogenetic Protein 7 Bone Morphogenetic Proteins / pharmacology Cell Differentiation* Cell Line DNA, Complementary / genetics Down-Regulation Epidermal Growth Factor / metabolism ErbB Receptors / analysis ErbB Receptors / genetics ErbB Receptors / physiology* Flow Cytometry Glycerophosphates / pharmacology Humans Integrin-Binding Sialoprotein Osteoblasts / cytology* Osteoblasts / drug effects Osteoblasts / metabolism Osteocalcin / genetics RNA, Messenger / analysis Sialoglycoproteins / genetics Transformation, Genetic Transforming Growth Factor beta / pharmacology |
| IF | 3.423 |
| 引用数 | 37 |
| WOS 分野 | ENDOCRINOLOGY & METABOLISM |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |