論文 - 詳細
| RRC ID | 39746 |
|---|---|
| 著者 | Mai JC, Coleman AW. |
| タイトル | The internal transcribed spacer 2 exhibits a common secondary structure in green algae and flowering plants. |
| ジャーナル | J Mol Evol |
| Abstract |
Sequences of the Internal Transcribed Spacer 2 (ITS-2) regions of the nuclear rDNA repeats from 111 organisms of the family Volvocaceae (Chlorophyta) and unicellular organisms of the Volvocales, including Chlamydomonas reinhardtii, were determined. The use of thermodynamic energy optimization to generate secondary structures and phylogenetic comparative analysis of the spacer regions revealed a common secondary structure that is conserved despite wide intra- and interfamilial primary sequence divergence. The existence of this conserved higher-order structure is supported by the presence of numerous compensating basepair changes as well as by an evolutionary history of insertions and deletions that nevertheless maintains major aspects of the overall structure. Furthermore, this general structure is preserved across broad phylogenetic lines, as it is observed in the ITS-2s of other chlorophytes, including flowering plants; previous reports of common ITS-2 secondary structures in other eukaryotes were restricted to the order level. The reported ITS-2 structure possesses important conserved structural motifs which may help to mediate cleavages in the ITS-2 that occur during rRNA transcript processing. Their recognition can guide further studies of eukaryotic rRNA processing, and their application to sequence alignments may contribute significantly to the value of ITS-2 sequences in phylogenetic analyses at several taxonomic levels, but particularly in characterizing populations and species. |
| 巻・号 | 44(3) |
| ページ | 258-71 |
| 公開日 | 1997-3-1 |
| DOI | 10.1007/pl00006143 |
| PMID | 9060392 |
| MeSH | Base Sequence Chlorophyta / classification Chlorophyta / genetics* DNA, Ribosomal / chemistry* Evolution, Molecular Molecular Sequence Data Nucleic Acid Conformation* Phylogeny Plants / classification Plants / genetics* |
| IF | 1.821 |
| 引用数 | 273 |
| WOS 分野 | EVOLUTIONARY BIOLOGY GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 藻類 | NIES-691 |