Reference - Detail
| RRC ID | 39801 |
|---|---|
| Author | Ohkawa K, Takada K, Asakura T, Hashizume Y, Okawa Y, Tashiro K, Ueda J, Itoh Y, Hibi N. |
| Title | Calpain inhibitor inhibits secretory granule maturation and secretion of GH. |
| Journal | Neuroreport |
| Abstract |
Clathrin- and AP-1-coated buds are present on immature secretory granules of endocrine cells that mature into clathrin-uncoated granules. The mechanism of clathrin and adaptor protein uncoating has remained obscure. Benzyloxycarbonyl-L-leucyl-L-leucinal (ZLLal), a calpain inhibitor, reduced growth hormone (GH) secretion with intracellular accumulation, in a GH-secreting rat pituitary tumor cell. Pulse and chase demonstrated that ZLLal retarded the turnover of clathrin (Clt.H) and adaptins. ZLLal-treatment co-immunoprecipitated the increased amounts of GH with Clt.H and adaptins compared to control cells, suggesting the intracellular accumulation of immature secretory granules. Clt.H and adaptins were limited-proteolyzed by m-calpain in vitro, indicating that calpain may be involved partly in the maturation of secretory granules in endocrine cells via the process of clathrin uncoating. |
| Volume | 11(18) |
| Pages | 4007-11 |
| Published | 2000-12-18 |
| DOI | 10.1097/00001756-200012180-00021 |
| PMID | 11192619 |
| MeSH | Adaptor Protein Complex alpha Subunits Adaptor Proteins, Vesicular Transport Animals Calpain / antagonists & inhibitors Calpain / metabolism* Clathrin / drug effects Clathrin / metabolism* Cysteine Proteinase Inhibitors / pharmacology Dipeptides / pharmacology Dose-Response Relationship, Drug Glycoproteins / pharmacology* Golgi Apparatus / drug effects Golgi Apparatus / metabolism Growth Hormone / metabolism* Growth Hormone-Releasing Hormone / pharmacology Membrane Proteins / drug effects Membrane Proteins / metabolism* Pituitary Gland, Anterior / drug effects Pituitary Gland, Anterior / metabolism* Pituitary Gland, Anterior / ultrastructure Rats Secretory Vesicles / drug effects Secretory Vesicles / metabolism* Tumor Cells, Cultured / drug effects Tumor Cells, Cultured / metabolism |
| IF | 1.394 |
| Times Cited | 12 |
| WOS Category | NEUROSCIENCES |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Resource | |
| Human and Animal Cells | MtT/S(RCB0528) |