論文 - 詳細
| RRC ID | 39804 |
|---|---|
| 著者 | Nagamune H, Maeda T, Ohkura K, Yamamoto K, Nakajima M, Kourai H. |
| タイトル | Evaluation of the cytotoxic effects of bis-quaternary ammonium antimicrobial reagents on human cells. |
| ジャーナル | Toxicol In Vitro |
| Abstract |
The cytotoxic effects of newly synthesized bis-quaternary ammonium compounds (bis-QACs) and benzalkonium chloride were investigated on skin and blood cells, namely normal human epidermal keratinocytes of neonatal foreskin, a normal human skin fibroblast cell line NB1RGB, erythrocytes and a lymphoma cell line JM. The bis-QACs tested were 4, 4'-(1,6-hexamethylenedithio)bis(1-octylpyridinium iodide) (4DTBP-6, 8), N,N'-tetramethylenebis(1-dodecyl-4-carbamoylpyridinium iodide) (4BCAP-4,12), N,N'-hexamethylenebis(1-decyl-4-carbamoylpyridinium iodide) (4BCAP-6,10), 4,4'-(1,4-tetramethylenedicarbonyldiamine) bis (1-decylpyridinium iodide) (4DCABP-4,10), 4,4'-(1, 4-tetramethylenedicarbonyldiamine) bis (1-dodecylpyridinium iodide) (4DCABP-4,12), 4,4'-(1, 4-phenyldicarbonyldiamine)bis(1-dodecylpyridinium iodide) (4DCABP-P, 12), and 4,4'-(1, 6-hexamethylenedioxydicarbonyl)bis(1-dodecylpyridinium iodide) (4DOCBP-6,12). All bis-QACs consisted of two identical alkylpyridinium rings and bridge structure linking rings to each other have a methylene bridge but only 4DCABP-P,12 has a phenyl ring as a bridge. Most of the LD(50) values in acute cytotoxic assays of these bis-QACs tended to be lower than those of benzalkonium chloride. However, the comparison of the antibacterial activity against cytotoxic effects on several human cells revealed that bis-QACs, especially 4DTBP-6,8, have wide concentration ranges showing sufficient antibacterial activity and lower cytotoxic effect on human cells (except for 4DOCBP-6,12), although benzalkonium chloride caused significant human cell damage at the concentrations necessary for antibacterial activity. Moreover, judging from the LD(50) value of 4DTBP-6,8 [67 microM, a 6.7-fold higher concentration than the upper value of MIC] obtained in an artificial human skin model TESTSKIN, 4DTBP-6,8 is thought to be a more effective and safer antiseptic reagent for application to the skin surface than benzalkonium chloride. Taken together, bis-QACs are expected to be a promising new generation of antimicrobial QACs. |
| 巻・号 | 14(2) |
| ページ | 139-47 |
| 公開日 | 2000-4-1 |
| DOI | 10.1016/s0887-2333(00)00003-5 |
| PII | S0887-2333(00)00003-5 |
| PMID | 10793292 |
| MeSH | Anti-Bacterial Agents / chemistry Anti-Bacterial Agents / toxicity* Bacteria / drug effects Benzalkonium Compounds / toxicity Cell Line Cell Survival / drug effects Erythrocytes / drug effects Fibroblasts Humans Indicators and Reagents Keratinocytes / drug effects Lymphocytes / drug effects Lymphoma, T-Cell / pathology Microbial Sensitivity Tests Preservatives, Pharmaceutical / toxicity Quaternary Ammonium Compounds / chemistry Quaternary Ammonium Compounds / toxicity* |
| IF | 2.959 |
| 引用数 | 70 |
| WOS 分野 | TOXICOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | NB1RCB(RCB0222) |