Reference - Detail
| RRC ID | 39827 |
|---|---|
| Author | Arai T, Abe K, Matsuoka H, Yoshida M, Mori M, Goya S, Kida H, Nishino K, Osaki T, Tachibana I, Kaneda Y, Hayashi S. |
| Title | Introduction of the interleukin-10 gene into mice inhibited bleomycin-induced lung injury in vivo. |
| Journal | Am J Physiol Lung Cell Mol Physiol |
| Abstract |
Interleukin (IL)-10 has been shown to reduce many inflammatory reactions. We investigated the in vivo effects of IL-10 on a bleomycin-induced lung injury model. Hemagglutinating virus of Japan (HVJ)-liposomes containing a human IL-10 expression vector (hIL10-HVJ) or a balanced salt solution as a control (Cont-HVJ) was intraperitoneally injected into mice on day -3. This was followed by intratracheal instillation of bleomycin (0.8 mg/kg) on day 0. Myeloperoxidase activity of bronchoalveolar lavage fluid and tumor necrosis factor-alpha mRNA expression in bronchoalveolar lavage fluid cells on day 7 and hydroxyproline content of the whole lung on day 21 were inhibited significantly by hIL10-HVJ treatment. However, Cont-HVJ treatment could not suppress any of these parameters. We also examined the in vitro effects of IL-10 on the human lung fibroblast cell line WI-38. IL-10 significantly reduced constitutive and transforming growth factor-beta-stimulated type I collagen mRNA expression. However, IL-10 did not affect the proliferation of WI-38 cells induced by platelet-derived growth factor. These data suggested that exogenous IL-10 may be useful in the treatment of pulmonary fibrosis. |
| Volume | 278(5) |
| Pages | L914-22 |
| Published | 2000-5-1 |
| DOI | 10.1152/ajplung.2000.278.5.L914 |
| PMID | 10781421 |
| MeSH | Animals Antibiotics, Antineoplastic Bleomycin Cell Division / drug effects Cells, Cultured Collagen / genetics Fibroblasts / immunology Fibroblasts / pathology Gene Expression / immunology Gene Transfer Techniques* Humans Interleukin-10 / genetics* Interleukin-10 / immunology Mice Mice, Inbred C57BL Platelet-Derived Growth Factor / pharmacology Pneumonia / chemically induced Pneumonia / pathology Pneumonia / prevention & control Pulmonary Fibrosis / chemically induced* Pulmonary Fibrosis / pathology Pulmonary Fibrosis / prevention & control RNA, Messenger / analysis Respirovirus / genetics Specific Pathogen-Free Organisms Transforming Growth Factor beta / genetics Tumor Necrosis Factor-alpha / genetics |
| IF | 4.418 |
| Times Cited | 97 |
| WOS Category | PHYSIOLOGY RESPIRATORY SYSTEM |
| Resource | |
| Human and Animal Cells | |