RRC ID 39890
Author Ikeda S, Hachisu R, Yamaguchi A, Gao YH, Okano T.
Title Radiation retards muscle differentiation but does not affect osteoblastic differentiation induced by bone morphogenetic protein-2 in C2C12 myoblasts.
Journal Int J Radiat Biol
Abstract PURPOSE:Effects of radiation on the differentiation of C2C12 myoblasts were studied using cell cultures in which the cells differentiated into either muscle cells or osteoblasts.
MATERIALS AND METHODS:A mouse skeletal myoblast cell line, C2C12, was cultured and irradiated at confluence. Muscle differentiation was estimated by counting the number of myosin heavy chain (MHC)-positive multinucleated myotubes. Osteoblast differentiation was analysed by measuring alkaline phosphatase (ALP) activity and osteocalcin production in C2C12 cells treated with recombinant human bone morphogenetic protein-2 (rhBMP-2).
RESULTS:Myotube formation in C2C12 cells was inhibited by 2 and 4 Gy irradiation, but recovered to non-irradiated control levels following further culture. In irradiated cultures, the number of MHC-positive mononucleated cells increased significantly. Irradiation did not, however, alter the induction of osteoblastic phenotypes induced by rhBMP-2 in C2C12. Irradiation also did not affect the expression of MyoD mRNA and myogenin mRNA in myogenic differentiation and induction of osteocalcin mRNA in osteoblastic differentiation.
CONCLUSIONS:During differentiation of C2C12 cells, myotube formation was delayed after irradiation, while induction of osteoblasts with BMP-2 was unaffected.
Volume 76(3)
Pages 403-11
Published 2000-3-1
DOI 10.1080/095530000138745
PMID 10757320
MeSH Alkaline Phosphatase / biosynthesis Animals Bone Morphogenetic Protein 2 Bone Morphogenetic Proteins / metabolism* Bone Morphogenetic Proteins / pharmacology Cell Differentiation / drug effects Cell Differentiation / radiation effects* Cell Line Humans Mice Muscle, Skeletal / cytology Muscle, Skeletal / drug effects Muscle, Skeletal / metabolism* Muscle, Skeletal / radiation effects* Myogenin / biosynthesis Myogenin / genetics Myosin Heavy Chains / biosynthesis Myosins / biosynthesis Myosins / genetics Osteoblasts / cytology Osteoblasts / enzymology* Osteocalcin / biosynthesis Osteocalcin / genetics RNA, Messenger / biosynthesis Recombinant Proteins / metabolism Recombinant Proteins / pharmacology Transforming Growth Factor beta*
IF 2.368
Times Cited 21
Human and Animal Cells C2C12(RCB0987)