RRC ID |
3990
|
Author |
Higashi K, Ishiga Y, Inagaki Y, Toyoda K, Shiraishi T, Ichinose Y.
|
Title |
Modulation of defense signal transduction by flagellin-induced WRKY41 transcription factor in Arabidopsis thaliana.
|
Journal |
Mol Genet Genomics
|
Abstract |
Flagellin, a component of the flagellar filament of Pseudomonas syringae pv. tabaci 6605 (Pta), induces hypersensitive reaction in its non-host Arabidopsis thaliana. We identified the WRKY41 gene, which belongs to a multigene family encoding WRKY plant-specific transcription factors, as one of the flagellin-inducible genes in A. thaliana. Expression of WRKY41 is induced by inoculation with the incompatible pathogen P. syringae pv. tomato DC3000 (Pto) possessing AvrRpt2 and the non-host pathogens Pta within 6-h after inoculation, but not by inoculation with the compatible Pto. Expression of WRKY41 was also induced by inoculation of A. thaliana with an hrp-type three secretion system (T3SS)-defective mutant of Pto, indicating that effectors produced by T3SS in the Pto wild-type suppress the activation of WRKY41. Arabidopsis overexpressing WRKY41 showed enhanced resistance to the Pto wild-type but increased susceptibility to Erwinia carotovora EC1. WRKY41-overexpressing Arabidopsis constitutively expresses the PR5 gene, but suppresses the methyl jasmonate-induced PDF1.2 gene expression. These results demonstrate that WRKY41 may be a key regulator in the cross talk of salicylic acid and jasmonic acid pathways.
|
Volume |
279(3)
|
Pages |
303-12
|
Published |
2008-3-1
|
DOI |
10.1007/s00438-007-0315-0
|
PMID |
18219494
|
MeSH |
Arabidopsis / drug effects*
Arabidopsis / genetics
Arabidopsis / metabolism*
Arabidopsis / microbiology
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism*
Base Sequence
Cloning, Molecular
DNA Primers / genetics
DNA, Plant / genetics
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism
Flagellin / toxicity*
Gene Expression / drug effects
Gene Expression Profiling
Genes, Plant
Pectobacterium carotovorum / pathogenicity
Plant Diseases / genetics
Plant Diseases / microbiology
Pseudomonas syringae / pathogenicity
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction / drug effects
Transcription Factors / genetics
Transcription Factors / metabolism*
|
IF |
2.797
|
Times Cited |
66
|
WOS Category
|
GENETICS & HEREDITY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
Resource |
Arabidopsis / Cultured plant cells, genes |
rpc00008 |