RRC ID 3990
Author Higashi K, Ishiga Y, Inagaki Y, Toyoda K, Shiraishi T, Ichinose Y.
Title Modulation of defense signal transduction by flagellin-induced WRKY41 transcription factor in Arabidopsis thaliana.
Journal Mol. Genet. Genomics
Abstract Flagellin, a component of the flagellar filament of Pseudomonas syringae pv. tabaci 6605 (Pta), induces hypersensitive reaction in its non-host Arabidopsis thaliana. We identified the WRKY41 gene, which belongs to a multigene family encoding WRKY plant-specific transcription factors, as one of the flagellin-inducible genes in A. thaliana. Expression of WRKY41 is induced by inoculation with the incompatible pathogen P. syringae pv. tomato DC3000 (Pto) possessing AvrRpt2 and the non-host pathogens Pta within 6-h after inoculation, but not by inoculation with the compatible Pto. Expression of WRKY41 was also induced by inoculation of A. thaliana with an hrp-type three secretion system (T3SS)-defective mutant of Pto, indicating that effectors produced by T3SS in the Pto wild-type suppress the activation of WRKY41. Arabidopsis overexpressing WRKY41 showed enhanced resistance to the Pto wild-type but increased susceptibility to Erwinia carotovora EC1. WRKY41-overexpressing Arabidopsis constitutively expresses the PR5 gene, but suppresses the methyl jasmonate-induced PDF1.2 gene expression. These results demonstrate that WRKY41 may be a key regulator in the cross talk of salicylic acid and jasmonic acid pathways.
Volume 279(3)
Pages 303-12
Published 2008-3
DOI 10.1007/s00438-007-0315-0
PMID 18219494
MeSH Arabidopsis / drug effects* Arabidopsis / genetics Arabidopsis / metabolism* Arabidopsis / microbiology Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Base Sequence Cloning, Molecular DNA Primers / genetics DNA, Plant / genetics DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Flagellin / toxicity* Gene Expression / drug effects Gene Expression Profiling Genes, Plant Pectobacterium carotovorum / pathogenicity Plant Diseases / genetics Plant Diseases / microbiology Pseudomonas syringae / pathogenicity Reverse Transcriptase Polymerase Chain Reaction Signal Transduction / drug effects Transcription Factors / genetics Transcription Factors / metabolism*
IF 2.879
Times Cited 42
WOS Category GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Arabidopsis / Cultured plant cells, genes rpc00008