RRC ID 39939
著者 Hattori N, Saito T, Yagyu T, Jiang BH, Kitagawa K, Inagaki C.
タイトル GH, GH receptor, GH secretagogue receptor, and ghrelin expression in human T cells, B cells, and neutrophils.
ジャーナル J Clin Endocrinol Metab
Abstract We examined GH and GH receptor expression in human leukemic cell lines and leukocytes of normal subjects to elucidate the cell types expressing GH and GH receptor, the individual variations of their expressions, their correlation and the relationships with serum IgG and IGF-I concentrations. In addition, the expression of GH secretagogue receptor, which enhances GH secretion from the anterior pituitary by synthetic GH secretagogues and that of its endogenous ligand, ghrelin, were also examined in these immune cells. GH expression in human leukemic cell lines was observed mainly in B cell lines at both the mRNA and protein level [3.8 +/- 0.2 pg/10(6) cells in Raji and 19.9 +/- 3.3 pg/10(6) cells in Daudi vs. negligible in T cell lines (Jurkat and Hut-78) and in myeloid cell lines (K-562 and HL-60)]. B cells in normal subjects were also found to be the major immune cells expressing GH mRNA, with significant individual variation. GH receptor mRNA expression was detectable in all human leukemic cell lines, although the expression level varied widely among the cell lines and was weaker than that in the liver. On the other hand, GH receptor mRNA expression was mainly found in B cells, with marked individual variation in normal subjects. There was a positive correlation between the mRNA expressions of GH and GH receptor in B cells of normal subjects (r = 0.89; P < 0.001). Single cell RT-PCR revealed that some B cells expressed both GH and GH receptor transcripts, and others expressed only GH. GH/GH receptor expression levels in B cells did not show any correlation with serum IgG and IGF-I levels in normal subjects. Expression of GH secretagogue receptor and ghrelin was detectable in all immune cells regardless of the maturity and cell types with great individual variations. In summary, GH secreted from B cells may act locally on their own receptors, and their variable expressions may be related to individual immune functions. Widespread distribution of ghrelin and GH secretagogue receptor in human immune cells may indicate unknown biological functions other than enhancing GH secretion in the immune system.
巻・号 86(9)
ページ 4284-91
公開日 2001-9-1
DOI 10.1210/jcem.86.9.7866
PMID 11549663
MeSH B-Lymphocytes / metabolism* Blotting, Northern Cells, Cultured Chromatography, Gel Cloning, Molecular Enzyme-Linked Immunosorbent Assay Ghrelin Human Growth Hormone / metabolism* Humans Immunoglobulin G / metabolism Insulin-Like Growth Factor I / metabolism Neutrophils / metabolism* Peptide Hormones* Peptides / metabolism* RNA, Messenger / biosynthesis Receptors, Cell Surface / metabolism* Receptors, G-Protein-Coupled* Receptors, Ghrelin Receptors, Somatotropin / metabolism* Reverse Transcriptase Polymerase Chain Reaction T-Lymphocytes / metabolism*
IF 5.399
引用数 256
WOS 分野 ENDOCRINOLOGY & METABOLISM
リソース情報
ヒト・動物細胞 HL60(RCB0041) K562(RCB0027)