論文 - 詳細
| RRC ID | 39943 |
|---|---|
| 著者 | Mizuno M, Okayama N, Kasugai K, Yoshida T, Miyata M, Nitta M, Kataoka H, Akita S, Joh T, Itoh M, Asai K, Kato T. |
| タイトル | Acid stimulates E-cadherin surface expression on gastric epithelial cells to stabilize barrier functions via influx of calcium. |
| ジャーナル | Eur J Gastroenterol Hepatol |
| Abstract |
BACKGROUND AND AIMS:E-cadherin, which is a [Ca2+]-dependent, homotypic cell-cell adhesion molecule, is expressed in gastrointestinal epithelial cells. Much has been learned about the down-regulation of E-cadherin expression in gastrointestinal tumours, Barrett's oesophageal dysplasia, and Crohn's disease, but the functions of this molecule in normal gastrointestinal mucosa are less known. METHODS:In this study, we investigated the relationship between E-cadherin expression and permeability using rat cultured gastric and intestinal epithelial cells following a 30-min exposure to various pH solutions. We also investigated the participation of [Ca2+] in these events. RESULTS:E-cadherin expression increased under acid (pH 4) but not alkali (pH 10 or 11) exposure only for gastric epithelial cells. Gastric epithelial permeability was maintained only against acid exposure while intestinal permeability increased under both conditions. Transient influx of [Ca2+] was only observed for gastric epithelial cells just after acid exposure. CONCLUSIONS:These findings suggest that E-cadherin expression on gastric epithelium stabilizes the epithelial barrier against acid, probably through influx of [Ca2+]. This event is thought to be one of the protective mechanisms in gastric mucosa against acid back-diffusion, which is one of the causes of peptic ulcer formation. |
| 巻・号 | 13(2) |
| ページ | 127-36 |
| 公開日 | 2001-2-1 |
| DOI | 10.1097/00042737-200102000-00007 |
| PMID | 11246611 |
| MeSH | Acids Alkalies Animals Blotting, Western Cadherins / analysis Cadherins / biosynthesis* Calcium / metabolism* Cell Membrane Permeability Cell Survival Cells, Cultured Epithelial Cells Gastric Mucosa / cytology Gastric Mucosa / metabolism* Hydrogen-Ion Concentration Intestinal Mucosa / cytology Intestinal Mucosa / metabolism Rats Reverse Transcriptase Polymerase Chain Reaction |
| IF | 2.251 |
| 引用数 | 8 |
| WOS 分野 | GASTROENTEROLOGY & HEPATOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | RGM1(RCB0876) |