論文 - 詳細
| RRC ID | 39948 |
|---|---|
| 著者 | Okada E, Murai Y, Matsui K, Isizawa S, Cheng C, Masuda M, Takano Y. |
| タイトル | Survivin expression in tumor cell nuclei is predictive of a favorable prognosis in gastric cancer patients. |
| ジャーナル | Cancer Lett |
| Abstract |
One hundred and thirty three gastric cancer cases were investigated immunohistochemically to clarify the biological role of survivin in gastric cancer cells using a commercially available anti-survivin antibody (SURV11A). Five gastric cancer cell lines were employed to assess localization of survivin by reverse-transcription-polymerase chain reaction (RT-PCR) Southern blotting, Western blotting and immunofluorescence, signals being found in both nucleus and cytoplasm. Survivin nuclear staining of gastric cancer cells was evident in 109 of 133 cases (82.0%) and associated with a favorable prognosis, being an independent prognosticator on multivariate analysis. Survivin nuclear positivity also correlated with younger age and lower incidence of vessel cancer invasion. In contrast, survivin cytoplasmic positivity was noted in 117 cases (88.0%) and did not correlate with any factor of progression or prognosis. The results indicate that survivin is present in the majority of gastric cancer cells but a nuclear localization may play an important physiological role in hindering tumor progression. |
| 巻・号 | 163(1) |
| ページ | 109-16 |
| 公開日 | 2001-2-10 |
| DOI | 10.1016/s0304-3835(00)00677-7 |
| PII | S0304383500006777 |
| PMID | 11163114 |
| MeSH | Adult Aged Blotting, Southern Blotting, Western Cell Fractionation Cell Nucleus / genetics* Cell Nucleus / metabolism DNA, Complementary / genetics Female Fluorescent Antibody Technique Gene Expression Regulation, Neoplastic Humans Immunohistochemistry Inhibitor of Apoptosis Proteins Male Microtubule-Associated Proteins* Middle Aged Neoplasm Proteins Predictive Value of Tests Prognosis Proteins / genetics* Proteins / metabolism RNA, Messenger / genetics RNA, Messenger / metabolism Reverse Transcriptase Polymerase Chain Reaction Stomach Neoplasms / genetics Stomach Neoplasms / mortality Stomach Neoplasms / pathology* Survival Analysis Survival Rate Survivin |
| IF | 7.36 |
| 引用数 | 156 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | MKN45(RCB1001) MKN28(RCB1000) HGC-27(RCB0500) HuG1-N(RCB1179) |